Article (Scientific journals)
A new method to induce oocyte maturation in holothuroids
Léonet, Aline; Rasolofonirina, R.; Wattiez, Ruddy et al.
2009In Invertebrate Reproduction and Development, 53 (1), p. 13-21
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Abstract :
[en] Oocyte maturation in sea cucumbers is stopped during the meiosis at prophase I. Maturation naturally concludes just before spawning leading to a mature oocyte ready to be fertilized. Although thermal shocks applied on mature individuals can induce spawning, it is currently not possible to perform reliably in vitro fertilization of sea cucumber oocytes. The present paper reports the discovery of a new and highly effective oocyte maturation inducer (OMI), called MIF (for Maturation Inducing Fractions). MIF's effects on oocytes of the marketable species Holothuria scabra were analysed and compared to that of the few OMIs described in literature. MIF induces the maturation and fertilization of more than 90% of oocytes while other OMIs [1-Methyladenine, dithiothreitol (DTT), dimercapto-propanol (BAL) and L-cysteine] induces 28 to 90% of oocytes to mature depending on the tested inducer. Moreover the use of the other OMIs results in fertilization rates that never exceed 40%, and the obtained larvae often present developmental abnormalities. MIF's action on H. scabra oocytes is efficient yearlong even outside the spawning period of H. scabra. MIF is effective on the oocytes of all aspidochirote species tested so far but only when it is prepared from spawns of regular female sea urchin.
Disciplines :
Biochemistry, biophysics & molecular biology
Biotechnology
Zoology
Author, co-author :
Léonet, Aline 
Rasolofonirina, R.
Wattiez, Ruddy  ;  Université de Mons > Faculté des Sciences > Protéomie et Microbiologie
Jangoux, Michel ;  Université de Mons > Faculté des Sciences > Biologie des Organismes Marins et Biomimétisme
Eeckhaut, Igor  ;  Université de Mons > Faculté des Sciences > Biologie des Organismes Marins et Biomimétisme
Language :
English
Title :
A new method to induce oocyte maturation in holothuroids
Publication date :
01 June 2009
Journal title :
Invertebrate Reproduction and Development
ISSN :
0792-4259
Publisher :
Taylor & Francis, United Kingdom
Volume :
53
Issue :
1
Pages :
13-21
Peer reviewed :
Peer Reviewed verified by ORBi
Research unit :
S828 - Protéomie et Microbiologie
S864 - Biologie des Organismes Marins et Biomimétisme
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