Adhesion/growth-regulatory Tissue Lectin Galectin-1 in Relation to Angiogenesis/lymphocyte Infiltration and Prognostic Relevance of Stromal Up-regulation in Laryngeal Carcinomas
[en] Background: Galectin-1 has been found to modulate lymphocyte invasion in inflammation and to be involved in angiogenesis in models, thus prompting examination of its clinical relevance in laryngeal cancer. Patients and Methods: Immunohistochemical processing of tissue sections (n=53) from patients with stage I/II (n=35) and stage IV (n=18) laryngeal squamous cell carcinoma (LSCC) with a specific anti-galectin-1 antibody and monitoring of CD45/CD31 positivity was combined with quantitative morphometric analysis. Results: Lectin presence in the tumor and endothelial cells was positively correlated, while a negative relationship to the number of CD45-positive lymphocytes was demonstrated. No association was seen with the extent of neovascularization. The mean optical density (MOD) of lectin-dependent staining in the tumor stroma was significantly increased compared to normal stroma. Conclusion: Galectin-1 was not associated with angiogenesis in the studied cohort while galectin-1 in endothelial cells may negatively influence lymphocyte invasion and the mean optical density for the stromal galectin-1 signal is up-regulated in tumors.
Disciplines :
Immunology & infectious disease Oncology
Author, co-author :
Saussez, Sven ; Université de Mons > Faculté de Médecine et de Pharmacie > Anatomie humaine et Oncologie expérimentale
Decaestecker, Christine
Cludts, Stéphanie
Ernoux, Perle
Chevalier, Dominique
Smetana, Karel
André, Sabine
Leroy, Xavier
Gabius, Hans-Joachim
Language :
English
Title :
Adhesion/growth-regulatory Tissue Lectin Galectin-1 in Relation to Angiogenesis/lymphocyte Infiltration and Prognostic Relevance of Stromal Up-regulation in Laryngeal Carcinomas
Publication date :
01 January 2009
Journal title :
Anticancer Research
ISSN :
0250-7005
Publisher :
International Institute of Anticancer Research, Greece
Volume :
29
Issue :
1
Pages :
59-65
Peer reviewed :
Peer Reviewed verified by ORBi
Research unit :
M112 - Anatomie humaine et Oncologie expérimentale
Gabius HJ (ed.): The Sugar Code. Fundamentals of Glycosciences. Weinheim, Wiley-VCH 2009.
Gabius HJ, Siebert HC, André S, Jiménez-Barbero J and Rüdiger H: The chemical biology of the sugar code. ChemBioChem 5: 740-764, 2004.
Villalobo A, Nogales-González A and Gabius HJ: A guide to signalling pathways connecting protein-glycan interaction with the emerging versatile effector functionality of mammalian lectins. Trends Glycosci Glycotechnol 18: 1-37, 2006.
Gabius HJ, Engelhardt R and Cramer F: Endogenous tumor lectins: overview and perspectives. Anticancer Res 6: 573-578, 1986.
Gabius HJ, Engelhardt R, Hellmann T, Midoux P, Monsigny M, Nagel GA and Vehmeyer K: Characterization of membrane lectins in human colon carcinoma cells by flow cytofluorometry, drug targeting and affinity chromatography. Anticancer Res 7: 109-112, 1987.
Bardosi A, Brkovic D and Gabius HJ: Localization of endogenous sugar-binding proteins (lectins) in tumors of the central and peripheral nervous system by biotinylated neoglycoproteins. Anticancer Res 11: 1183-1188, 1991.
Rorive S, Belot N, Decaestecker C, Lefranc F, Gordower L, Micik S, Maurage CA, Kaltner H, Ruchoux MM, Danguy A, Gabius HJ, Salmon I, Kiss R and Camby I: Galectin-1 is highly expressed in human gliomas with relevance for modulation of invasion of tumor astrocytes into the brain parenchyma. GLIA 33: 241-255, 2001.
André S, Kaltner H, Lensch M, Russwurm R, Siebert HC, Fallsehr C, Tajkhorshid E, Heck AJR, von Knebel Doeberitz M, Gabius HJ and Kopitz J: Determination of structural and functional overlap/divergence of five proto-type galectins by analysis of the growth-regulatory interaction with ganglioside GM1 in silica and in vitro on human neuroblastoma cells. Int J Cancer 114: 46-57, 2005.
Fischer C, Sanchez-Ruderisch H, Welzel M, Wiedenmann B, Sakai T, André S, Gabius HJ, Khachigian L, Detjen KM and Rosewicz S: Galectin-1 interacts with the α5β1 fibronectin receptor to restrict carcinoma cell growth via induction of p21 and p27. J Biol Chem 280: 37266-37277, 2005.
André S, Sanchez-Ruderisch H, Nakagawa H, Buchholz M, Kopitz J, Forberich P, Kemmner W, Bock C, Deguchi K, Detjen KM, Wiedenmann B, von Knebel Doeberitz M, Gress TM, Nishimura SI, Rosewicz S and Gabius HJ: Tumor suppressor p16INK4a-modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells. FEBS J 274: 3233-3256, 2007.
Rotblat B, Niv H, André S, Kaltner H, Gabius HJ and Kloog Y: Galectin-1 (L11A) predicted from a computed galectin-1 farnesyl-binding pocket selectively inhibits Ras-GTP. Cancer Res 64: 3112-3118, 2004.
Smetana K Jr, Dvoránková B, Chovanec M, Boucek J, Klíma J, Motlík J, Lensch M, Kaltner H, André S and Gabius HJ: Nuclear presence of adhesion-/growth-regulatory galectins in normal/ malignant cells of squamous epithelial origin. Histochem Cell Biol 125: 171-182, 2006.
Nagy N, Legendre H, Engels O, André S, Kaltner H, Wasano K, Zick Y, Pector JC, Decaestecker C, Gabius HJ, Salmon I and Kiss R: Refined prognostic evaluation in colon carcinoma using immunohistochemical galectin fingerprinting. Cancer 97: 1849-1858, 2003.
Langbein S, Brade J, Badawi JK, Hatzinger M, Kaltner H, Lensch M, Specht K, André S, Brinck U, Alken P and Gabius HJ: Gene-expression signature of adhesion/growth-regulatory tissue lectins (galectins) in transitional cell cancer and its prognostic relevance. Histopathology 51: 681-690, 2007.
Dettmann W, Grandbois M, André S, Benoit M, Wehle AK, Kaltner H, Gabius HJ and Gaub HE: Differences in zero-force and force-driven kinetics of ligand dissociation from β-galactoside-specific proteins (plant and animal lectins, immunoglobulin G) monitored by plasmon resonance and dynamic single molecule force microscopy. Arch Biochem Biophys 383: 157-170, 2000.
Siebert HC, André S, Lu SY, Frank M, Kaltner H, van Kuik JA, Korchagina EY, Bovin NV, Tajkhorshid E, Kaptein R, Vliegenthart JFG, von der Lieth CW, Jiménez-Barbero J, Kopitz J and Gabius HJ: Unique conformer selection of human growth-regulatory lectin galectin-1 for ganglioside GM1 versus bacterial toxins. Biochemistry 42: 14762-14773, 2003.
López-Lucendo MF, Solís D, André S, Hirabayashi J, Kasai K-I, Kaltner H, Gabius HJ and Romero A: Growth-regulatory human galectin-1: crystallographic characterisation of the structural changes induced by single-site mutations and their impact on the thermodynamics of ligand binding. J Mol Biol 343: 957-970, 2004.
Dam TK, Gabius HJ, André S, Kaltner H, Lensch M and Brewer CF: Galectins bind to the multivalent glycoprotein asialofetuin with enhanced affinities and a gradient of decreasing binding constants. Biochemistry 44: 12564-12571, 2005.
Debbage PL, Gabius HJ, Bise K and Marguth F: Cellular glycoconjugates and their potential endogenous receptors in the cerebral microvasculature of man: a glycohistochemical study. Eur J Cell Biol 46: 425-434, 1988.
Lotan R, Belloni PN, Tressler RJ, Lotan D, Xu XC and Nicolson GL: Expression of galectins on microvessel endothelial cells and their involvement in tumour cell adhesion. Glycoconj J 11: 462-468, 1994.
Baum LG, Seilhamer JJ, Pang M, Levine WB, Beynon D and Berliner JA: Synthesis of an endogeneous lectin, galectin-1, by human endothelial cells is up-regulated by endothelial cell activation. Glycoconj J 12: 63-68, 1995.
La M, Cao TV, Cerchiaro G, Chilton K, Hirabayashi J, Kasai K-i, Oliani SM, Chernajovsky Y and Perretti M: A novel biological activity for galectin-1: inhibition of leukocyte-endothelial cell interactions in experimental inflammation. Am J Pathol 163: 1505-1515, 2003.
Thijssen VLJL, Poirier F, Baum LG and Griffioen AW: Galectins in the tumor endothelium: opportunities for combined cancer therapy. Blood 110: 2819-2827, 2007.
Norling LV, Sampaio ALF, Cooper D and Perretti M: Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking. FASEB J 22: 682-690, 2008.
Saussez S, Cucu DR, Decaestecker C, Chevalier D, Kaltner H, André S, Wacreniez A, Toubeau G, Camby I, Gabius HJ and Kiss R: Galectin-7 (p53-induced gene 1): a new prognostic predictor of recurrence and survival in stage IV hypopharyngeal cancer. Ann Surg Oncol 13: 999-1009, 2006.
Saussez S, Decaestecker C, Lorfevre F, Cucu DR, Mortuaire G, Chevalier D, Wacreniez A, Kaltner H, André S, Toubeau G, Camby I, Gabius HJ and Kiss R: High level of galectin-1 expression is a negative prognostic predictor of recurrence rn laryngeal squamous cell carcinomas. Int J Oncol 30: 1109-1117, 2007.
Saussez S, Decaestecker C, Lorfevre F, Chevalier D, Mortuaire G, Kaltner H, André S, Toubeau G, Gabius HJ and Leroy X: Increased expression and altered intracellular distribution of adhesion/growth-regulatory lectins galectins-1 and -7 during tumour progression in hypopharyngeal and laryngeal squamous cell carcinomas. Histopathology 52: 483-493, 2008.
Hyams VJ, Batsakis JG and Michaels L (eds.): Tumors of the Upper Respiratory Tract and Ear. Atlas of Tumor Pathology. Washington, DC: Armed Forces Institute of Pathology, 130, 1988.
Wittekind C, Greene FL, Hutter RVP, Klimpfinger M and Sobin LH: UICC TNM Atlas. 5th Edition. UICC Springer, pp. 22-50, 2004.
Lohr M, Lensch M, André S, Kaltner H, Siebert HC, Smetana K Jr, Sinowatz F and Gabius HJ: Murine homodimeric adhesion/growth-regulatory galectins-1, -2 and -7: comparative profiling of gene/promoter sequences by database mining, of expression by RT-PCR/immunohistochemistry and of contact sites for carbohydrate ligands by computational chemistry. Folia Biol (Praha) 53: 109-128, 2007.
Zubieta MR, Furman MB, Bravo AI, Domenichini E and Mordoh J: Galectin-3 expression correlates with apoptosis of tumor-associated lymphocytes in human melanoma biopsies. Am J Pathol 168: 1666-1675, 2006.
Rappl G, Abken H, Muche JM, Sterry W, Tilgen W, André S, Kaltner H, Ugurel S, Gabius HJ and Reinhold U: CD4+CD7-leukemic T-cells from patients with Sézary syndrome are protected from galectin-1-triggered T-cell death. Leukemia 16: 840-845, 2002.
Moisa A, Fritz P, Eck A, Wehner HD, Mürdter T, Simon W and Gabius HJ: Growth/adhesion-regulatory tissue lectin galectin-3: stromal presence but not cytoplasmic/nuclear expression in tumor cells as a negative prognostic factor in breast cancer. Anticancer Res 27: 2131-2139, 2007.
Purkrábková T, Smetana K Jr, Dvoránková B, Holíková Z, Böck C, Lensch M, André S, Pytlík R, Liu FT, Klíma J, Smetana K, Motlík J and Gabius HJ: New aspects of galectin functionality in nuclei of cultured bone marrow stromal and epidermal cells: biotinylated galectins as tool to detect specific binding sites. Biol Cell 95: 535-545, 2003.
Saussez S, Lorfevre F, Nonclercq D, Laurent G, André S, Journé F, Kiss R, Toubeau G and Gabius HJ: Towards functional glycomics by localization of binding sites for tissue lectins: lectin histochemical reactivity for galectins during diethylstilbestrol-induced kidney tumorigenesis in male Syrian hamster. Histochem Cell Biol 126: 57-69, 2006.
Lohr M, Kaltner H, Lensch M, André S, Sinowatz F and Gabius HJ: Cell-type-specific expression of murine multifunctional galectin-3 and its association with follicular atresia/luteolysis in contrast to pro-apoptotic galectins-1 and -7. Histochem Cell Biol 130: 567-581, 2008.
André S, Kaltner H, Furuike T, Nishimura SI and Gabius HJ: Persubstituted cyclodextrin-based glycoclusters as inhibitors of protein-carbohydrate recognition using purified plant and mammalian lectins and wild-type and lectin-gene-transfected tumor cells as targets. Bioconjug Chem 15: 87-98, 2004.
André S, Pei Z, Siebert HC, Ramström O and Gabius HJ: Glycosyldisulfides from dynamic combinatorial libraries as O-glycoside mimetics for plant and endogenous lectins: their reactivities in solid-phase and cell assays and conformational analysis by molecular dynamics simulations. Bioorg Med Chem 14: 6314-6326, 2006.
André S, Maljaars CEP, Halkes KM, Gabius HJ and Kamerling JP: Discovery of galectin ligands in fully randomized combinatorial one-bead-one-compound (glyco)peptide libraries. Bioorg Med Chem Lett 17: 793-798, 2007.
André S, Sansone F, Kaltner H, Casnati A, Kopitz J, Gabius HJ and Ungaro R: Calix[n]arene-based glycoclusters: bioactivity of thiourea-linked galactose/lactose moieties as inhibitors of binding of medically relevant lectins to a glycoprotein and cell-surface glycoconjugates and selectivity among human adhesion/growth-regulatory galectins. ChemBioChem 9: 1649-1661, 2008.
