Article (Scientific journals)
Binding of a 100-kDa ubiquitous factor to the human prolactin promoter is required for its basal and hormone-regulated activity.
Peers, B; Nalda, A-M; Monget, P et al.
1992In European Journal of Biochemistry, 210 (1), p. 53-58
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Abstract :
[en] cAMP strongly stimulates the activity of the human prolactin (hPRL) promoter. We have previously shown that two types of cis-element are required for this cAMP regulation; binding sites for the pituitary-specific factor Pit-1, and the sequence spanning nucleotides -115 to -85 (named sequence A). Sequence A contains the TGACG motif found in the consensus sequence of the cAMP-responsive element (CRE). In this study, we show that a mutation in the TGACG motif of sequence A strongly reduces not only the cAMP regulation but also the Ca2+ regulation and basal activity of the hPRL promoter. Furthermore, gel-shift assays indicate that the mutation prevents binding of a ubiquitous factor which is not the CRE-binding protein. Southwestern experiments suggest that this ubiquitous factor's molecular mass is approximately 100 kDa. We conclude that binding of a 100-kDa ubiquitous factor to sequence A is required for full basal and hormonal regulation of hPRL-promoter activity.
Disciplines :
Biotechnology
Author, co-author :
Peers, B
Nalda, A-M
Monget, P
Voz, ML
Belayew, Alexandra 
Martial, JA
Language :
English
Title :
Binding of a 100-kDa ubiquitous factor to the human prolactin promoter is required for its basal and hormone-regulated activity.
Publication date :
01 November 1992
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
210
Issue :
1
Pages :
53-58
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi UMONS :
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