Psychiatry and Mental health; Geriatrics and Gerontology; Clinical Psychology; General Neuroscience
Abstract :
[en] Background: Alzheimer’s disease (AD) is a neurodegenerative disorder lacking any curative treatment up to now. Indeed, actual medication given to the patients alleviates only symptoms. The cytosolic phospholipase A2 (cPLA2-IVA) appears as a pivotal player situated at the center of pathological pathways leading to AD and its inhibition could be a promising therapeutic approach. Objective: A cPLA2-IVA inhibiting peptide was identified in the present work, aiming to develop an original therapeutic strategy. Methods: We targeted the cPLA2-IVA using the phage display technology. The hit peptide PLP25 was first validated in vitro (arachidonic acid dosage [AA], cPLA2-IVA cellular translocation) before being tested in vivo. We evaluated spatial memory using the Barnes maze, amyloid deposits by MRI and immunohistochemistry (IHC), and other important biomarkers such as the cPLA2-IVA itself, the NMDA receptor, AβPP and tau by IHC after i.v. injection in APP/PS1 mice. Results: Showing a high affinity for the C2 domain of this enzyme, the peptide PLP25 exhibited an inhibitory effect on cPLA2-IVA activity by blocking its binding to its substrate, resulting in a decreased release of AA. Coupled to a vector peptide (LRPep2) in order to optimize brain access, we showed an improvement of cognitive abilities of APP/PS1 mice, which also exhibited a decreased number of amyloid plaques, a restored expression of cPLA2-IVA, and a favorable effect on NMDA receptor expression and tau protein phosphorylation. Conclusions: cPLA2-IVA inhibition through PLP25 peptide could be a promising therapeutic strategy for AD.
Research center :
CMMI - Centre de Recherche en Microscopie et Imagerie Médicale
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Andre, Séverine ; Université de Mons - UMONS > Faculté de Médecine et de Pharmacie > Service de Chimie générale, organique et biomédicale
Verteneuil, Sébastien; General, Organic and Biomedical Chemistry Unit, NMR and Molecular Imaging Laboratory, University of Mons, Mons, Belgium
Ris, Laurence ; Université de Mons - UMONS > Faculté de Médecine et de Pharmac > Service de Neurosciences
Kahvecioglu, Zehra Cagla ; UMONS - Université de Mons [BE] > FMP > Service de Chimie Générale, Organique et Biomédicale
Nonclercq, Denis ; Université de Mons - UMONS > Faculté de Médecine et de Pharmac > Service d'Histologie
De winter, Julien ; Université de Mons - UMONS > Faculté des Science > Service de Synthèse et spectrométrie de masse organiques
Vander elst, Luce ; Université de Mons - UMONS > Faculté de Médecine et de Pharmacie > Service du Doyen de la Faculté de Médecine et Pharmacie
Laurent, Sophie ; Université de Mons - UMONS > Faculté de Médecine et de Pharmac > Service de Chimie générale, organique et biomédicale ; Center for Microscopy and Molecular Imaging, Gosselies, Belgium
Muller, Robert ; Université de Mons - UMONS > Faculté de Médecine et de Pharmac > Service du Doyen de la Faculté de Médecine et Pharmacie ; Center for Microscopy and Molecular Imaging, Gosselies, Belgium
Burtea, Carmen ; Université de Mons - UMONS > Faculté de Médecine et de Pharmac > Service de Chimie générale, organique et biomédicale
Language :
English
Title :
Modulation of Cytosolic Phospholipase A2 as a Potential Therapeutic Strategy for Alzheimer’s Disease
M108 - Chimie générale, organique et biomédicale M119 - Neurosciences M118 - Histologie Organic Synthesis and Mass Spectrometry
Research institute :
R550 - Institut des Sciences et Technologies de la Santé R100 - Institut des Biosciences
Name of the research project :
3681 - FRMH 2016 BURTEA - Développement d'une stratégie moléculaire de traitement de la maladie d'Alzheimer via la modulation d'une isoforme de la phospholipase. - Sources privées 132 - ARC UMONS 5 - identification de nouveaux outils diagnostiques d'imagerie moléculaires basés sur la caractérisation de la réponse cellulaire aux signaux de mort - Fédération Wallonie Bruxelles 3256 - Fondation Rotary 2015 André - Development of a molecular strategy for Alzheimer's disease therapy by modulation of a phospholipase A2 isoform - Sources privées
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