[en] The rationale and objectives of the study were to evaluate the influence of the experimental conditions (buffer, salt, etc.) on the data characterizing the non-covalent interaction between MRI contrast agents and human serum albumin and hence their in vivo relaxivity. The interaction of three gadolinium contrast agents (Gd-EOB-DTPA, Gd-BOPTA and MP-2269) with human serum albumin was assessed through the measurement of proton relaxation rate enhancement in various experimental conditions. The data show the negative effect of halide salts on the paramagnetic relaxation enhancement of the three contrast agents. The presence of halide salts can thus have a negative effect on the efficacy of MRI contrast agents interacting with HSA. In addition., careful attention must be paid to comparisons of the binding parameters of various contrast agents reported in different studies since the composition of the medium can greatly influence the non-covalent interaction.
Disciplines :
Radiology, nuclear medicine & imaging
Author, co-author :
Henrotte, Virginie
Muller, Robert ; Université de Mons > Faculté de Médecine et de Pharmacie > Chimie générale, organique et biomédicale
Bartholet, A.
Vander Elst, Luce ; Université de Mons > Faculté de Médecine et de Pharmacie > Biochimie métabolique et moléculaire
Language :
English
Title :
The presence of halide salts influences the non-covalent interaction of MRI contrast agents and human serum albumin
Publication date :
01 January 2007
Journal title :
Contrast Media and Molecular Imaging
ISSN :
1555-4309
Publisher :
John Wiley & Sons, Hoboken, United States - New Jersey
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