Unpublished conference/Abstract (Scientific congresses and symposiums)
Monitoring and modelling of E. coli BL21(DE3)
Retamal, Cristina; Dewasme, Laurent; Hantson, Anne-Lise et al.
201332th Benelux Meeting on Systems and Control
 

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Abstract :
[en] Escherichia coli is one of most used host microorganisms in biotechnology for the production of a large variety of indus- trially relevant components, such as recombinant proteins, insulin and growth hormone. However, the production of acetate during the culture, a phenomenon which is known as 'Overflow metabolism', inhibits the cell growth. Fed-batch mode is used in E. coli cultures because it allows to reduce acetate formation by manipulation of the feed-stream. An intuitive regulation strategy may be to control the acetate concentration in the culture media at a very low value. However, there is no acetate probe on the market allowing on-line measurements. In this work, state estimation of the acetate concentration of a BL21(DE3) E. coli strain is presented. For this purpose, an identified model is needed. Hence, pa- rameter identification of the mechanistic model of E. coli is carried out, as well.
Research center :
BIOSYS - Biosys
Disciplines :
Electrical & electronics engineering
Biotechnology
Author, co-author :
Retamal, Cristina 
Dewasme, Laurent  ;  Université de Mons > Faculté Polytechnique > Systèmes, Estimation, Commande et Optimisation
Hantson, Anne-Lise  ;  Université de Mons > Faculté Polytechnique > Chimie et Biochimie appliquées
Vande Wouwer, Alain  ;  Université de Mons > Faculté Polytechnique > Systèmes, Estimation, Commande et Optimisation
Language :
English
Title :
Monitoring and modelling of E. coli BL21(DE3)
Publication date :
26 March 2013
Number of pages :
1
Event name :
32th Benelux Meeting on Systems and Control
Event place :
Houffalize, Belgium
Event date :
2013
Research unit :
F504 - Chimie et Biochimie appliquées
F107 - Systèmes, Estimation, Commande et Optimisation
Research institute :
R100 - Institut des Biosciences
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