Influence of DNA condensation state on transfection efficiency in DNA/polymer complexes : an AFM and DLS comparative  study

Volcke, C.; Pirotton, S.; Grandfils, Ch.; Humbert, C; Thiry, P.A.; Ydens, I.; Dubois, Philippe; Raes, M.

doi:10.1016/j.jbiotec.2006.02.010

Article (Scientific journals)

Influence of DNA condensation state on transfection efficiency in DNA/polymer complexes : an AFM and DLS comparative study

Volcke, C.; Pirotton, S.; Grandfils, Ch. et al.

2006 • In Journal of Biotechnology, 125 (1), p. 11-21

Peer Reviewed verified by ORBi

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https://hdl.handle.net/20.500.12907/14108

DOI
10.1016/j.jbiotec.2006.02.010

PubMed
16860705

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Abstract :

[en] Atomic force microscopy (AFM) is used to describe the formation process of polymer/DNA complexes. Two main objectives of this research are presented. The first one is to apply AFM as an effective tool to analyse DNA molecules and different polycation/DNA complexes in order to evaluate their degree of condensation (size and shape). The other one is to search for a relationship between the condensation state of DNA and its transfection efficiency. In this study, linear methacrylate based polymers and globular SuperFect polymers are used in order to induce DNA condensation. Ternary complexes, composed of methacrylate based polymers and polyethylene glycol (PEG)-based copolymers, are also investigated. AFM allows us to confirm good condensation conditions and relate them (or not) to transfection efficiencies. These AFM results (obtained after drying in air) are compared with measurements deduced from Dynamic Light Scattering (DLS) experiments performed in water. This comparison allowed us to identify the structural modifications resulting from deposition on the mica surface.

Disciplines :

Chemistry

Author, co-author :

Volcke, C.

Pirotton, S.

Grandfils, Ch.

Humbert, C

Thiry, P.A.

Ydens, I.

Dubois, Philippe ; Université de Mons > Faculté des Sciences > Matériaux Polymères et Composites

Raes, M.

Language :

English

Title :

Influence of DNA condensation state on transfection efficiency in DNA/polymer complexes : an AFM and DLS comparative study

Publication date :

20 August 2006

Journal title :

Journal of Biotechnology

ISSN :

0168-1656

eISSN :

1873-4863

Publisher :

Elsevier, Netherlands

Volume :

125

Issue :

Pages :

11-21

Peer reviewed :

Peer Reviewed verified by ORBi

Research unit :

S816 - Matériaux Polymères et Composites

Research institute :

R400 - Institut de Recherche en Science et Ingénierie des Matériaux

Commentary :

Publié en ligne le 27 juin 2006 Lecture en ligne: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T3C-4K8S5F7-1-P&_cdi=4943&_user=532054&_pii=S0168165606001660&_origin=search&_coverDate=08%2F20%2F2006&_sk=998749998&view=c&wchp=dGLbVtb-zSkWA&md5=bd44070c436e7e5a2ad66a172eab7156

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