The characteristics of LTP induced in hippocampal slices are dependent on slice-recovery conditions

[en] In area CA1 of hippocampal slices which are allowed to recover from slicing 'in interface' and where recordings are carried out in interface, a single 1-sec train of 100-Hz stimulation triggers a short-lasting long-term potentiation (S-LTP), which lasts 1-2 h, whereas multiple 1-sec trains induce a long-lasting LTP (L-LTP), which lasts several hours.Moreover, the threshold and the features of these LTP depend on the history of the neurons, a phenomenon known as metaplasticity. Here, where all recordings were performed in interface, we found that allowing the slices to recover 'in submersion' had dramatic metaplastic effects. In these conditions, a single 1-sec train at 100 Hz induced an L-LTP which lasted at least 4 h and was dependent on protein synthesis. Interestingly, this type of metaplasticity was observed when the concentration of Mg++ used was 1.0 mM but not when it was 1.3 mM. The LTP induced by four 1-sec trains at 100 Hz was similar whatever the incubation method. However, the signaling cascades recruited to achieve that pattern were different. In the interface-interface paradigm (recovery and recording both in interface) the four-train induced LTP recruited the PKA signaling pathway but not that of the p42/44MAPK. On the contrary, in the submersion-interface paradigm the four-train induced LTP recruited the p42/44MAPK signaling pathway but not that of the PKA. To our knowledge this is the first example of metaplasticity involving the recruitment of signaling cascades in LTP.

Disciplines :

Neurology

Author, co-author :

Capron, Brigitte

Sindic, Christian

Godaux, Emile ; Université de Mons > Faculté de Médecine et de Pharmacie > Neurosciences

Ris, Laurence

Language :

English

Title :

The characteristics of LTP induced in hippocampal slices are dependent on slice-recovery conditions

Publication date :

01 May 2006

Journal title :

Learning and Memory

ISSN :

1072-0502

Publisher :

Cold Spring Harbor Laboratory Press, United States - New York

Volume :

Issue :

Pages :

271-277

Peer reviewed :

Peer Reviewed verified by ORBi

Research unit :

M119 - Neurosciences
M125 - Biologie humaine et Toxicologie

Available on ORBi UMONS :

since 10 June 2010

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