Article (Scientific journals)
Probing the interaction between DNA and cells transfection polymers with luminescent Ru(II) complexes
Kovbasyuk, L.; Moucheron, Cécile; Dubois, Philippe et al.
2009In New Journal of Chemistry, 33 (5), p. 1047-1056
Peer Reviewed verified by ORBi
 

Files


Full Text
356 Dubois et al New J Chem 2009 33 1047_1056.pdf
Author postprint (845.43 kB)
Download

All documents in ORBi UMONS are protected by a user license.

Send to



Details



Abstract :
[en] The luminescence properties of RuII complexes [Ru(TAP)2(phen)]Cl2 (1), [Ru(TAP)2(TPAC)]Cl2 (2) and [Ru(phen)2(TPAC)]Cl2 (3) (TAP = 1,4,5,8-tetraazaphenanthrene and TPAC = tetrapyrido-acridine) were used for probing the interactions of cell transfection (co)polymers, such as poly[2-dimethylaminoethyl-methacrylate] (PDMAEMA) and poly[2-dimethylaminoethylmethacrylate]-b-poly[(ethylene glycol-a-methylether-?-methacrylate] (P(DMAEMA-b-MAPEG)), with different polynucleotides (poly[(dA-dT)]2 and poly[(dG-dC)]2) and nucleic acids (calf thymus DNA and pBR322 plasmid DNA). It turned out that 1 was not a useful probe because its affinity constant for DNA was too weak. Moreover, 3 was also excluded because DNA-Ru complex aggregates complicated the interpretation of the data. 2 was the only candidate that could be used as a luminescent probe of 'DNA?polymer' self-assemblies that would penetrate cell membranes. Different (co)polymer/polynucleotide ratios were tested with complex 2 by luminescence measurements. The results showed that 2 is an interesting probe, which is very sensitive to changes in the polynucleotide double helix structure that are induced by interactions with the synthetic (co)polymer. Moreover, complex 2 has a mode of action of emission that is different from that of the classically used ethidium bromide, i.e. an increase in emission when certain 'polymer/DNA' ratios are reached during the titration of DNA with the polymer.
Disciplines :
Chemistry
Author, co-author :
Kovbasyuk, L.
Moucheron, Cécile
Dubois, Philippe  ;  Université de Mons > Faculté des Sciences > Matériaux Polymères et Composites
Kirsch-De-Mesmaeker, A.
Language :
English
Title :
Probing the interaction between DNA and cells transfection polymers with luminescent Ru(II) complexes
Publication date :
01 January 2009
Journal title :
New Journal of Chemistry
ISSN :
1144-0546
eISSN :
1369-9261
Publisher :
Royal Society of Chemistry, United Kingdom
Volume :
33
Issue :
5
Pages :
1047-1056
Peer reviewed :
Peer Reviewed verified by ORBi
Research unit :
S816 - Matériaux Polymères et Composites
Research institute :
R400 - Institut de Recherche en Science et Ingénierie des Matériaux
Available on ORBi UMONS :
since 10 July 2010

Statistics


Number of views
5 (0 by UMONS)
Number of downloads
22 (0 by UMONS)

Scopus citations®
 
1
Scopus citations®
without self-citations
1
OpenCitations
 
1
OpenAlex citations
 
1

Bibliography


Similar publications



Contact ORBi UMONS