[en] Oligomerization of linearized plasmids by nuclear proteins extracts, a recognized measure of nonhomologous end-joining (NHEJ) repair capacity, is typically assessed through agarose gel electrophoresis, a labor-intensive procedure. In the current study, a more convenient NHEJ assay was developed using microchips that allow scaled-down separation and quantification. This microchip method allows a considerable reduction in sample amount and analysis time with similar costs and comparable or slightly better precision. Data obtained with quercetin and wortmannin show that the method can be applied to the screening of food components and natural products for positive and negative modulators of NHEJ, potential chemopreventive and indirect genotoxic compounds, respectively.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Charles, Catherine ; Université de Mons > Faculté de Médecine et de Pharmacie > Chimie thérapeutique et Pharmacognosie
Ouedraogo, Moustapha
Belayew, Alexandra ; Université de Mons > Faculté de Médecine et de Pharmacie > Biochimie métabolique et moléculaire
Duez, Pierre ; Université de Mons > Faculté de Médecine et de Pharmacie > Chimie thérapeutique et Pharmacognosie
Language :
English
Title :
On-chips microelectrophoresis for the study of in vitro non-homologous end-joining DNA double-strand break repair