[en] Purple non-sulfur bacteria (PNSB) are known for their metabolic versatility and thrive as anoxygenic photoheterotrophs. In environmental engineering and resource recovery, cells would grow on mixtures of volatile fatty acids (VFA) generated by anaerobic fermentation of waste streams. In this study, we aim to better understand the behavior of Rhodospirillum rubrum, a model PNSB species, grown using multiple VFA as carbon sources. We highlighted that assimilation of individual VFA follows a sequential pattern. Based on observations in other PNSB, this seems to be specific to isocitrate lyase-lacking organisms. We hypothesized that the inhibition phenomenon could be due to the regulation of the metabolic fluxes in the substrate cycle between acetoacetyl-CoA and crotonyl-CoA. Developed macroscopic dynamic models showed a good predictive capability for substrate competition for every VFA mixture containing acetate, propionate, and/or butyrate. These novel insights provide valuable input for better design and operation of PNSB-based waste treatment solutions.
Disciplines :
Chemical engineering Electrical & electronics engineering Biotechnology Microbiology
Author, co-author :
Cabecas Segura, Paloma; UMONS - Université de Mons [BE] > Faculté des Sciences > Protéomie et Microbiologie
De Meur, Quentin; QUALITY ASSISTANCE S.A.
Alloul, Abbas; UA - University of Antwerp [BE] > Bioscience Engineering > Research Group of Sustainable Energy, Air and Water Technology
Tanghe, Audrey ; Université de Mons - UMONS > Unités externes > Materia Nova ASBL
Onderwater, Robertus ; Université de Mons - UMONS > Unités externes > Materia Nova ASBL
Vlaeminck, Siegfried E.; UA - University of Antwerp [BE] > Bioscience Engineering > Research Group of Sustainable Energy, Air and Water Technology
Vande Wouwer, Alain ; Université de Mons - UMONS > Faculté Polytechnique > Service Systèmes, Estimation, Commande et Optimisation
Wattiez, Ruddy ; Université de Mons - UMONS > Faculté des Sciences > Service de Protéomie et Microbiologie
Dewasme, Laurent ; Université de Mons - UMONS > Faculté Polytechnique > Service Systèmes, Estimation, Commande et Optimisation
Leroy, Baptiste ; Université de Mons - UMONS > Faculté des Sciences > Service de Protéomie et Microbiologie
Language :
English
Title :
Preferential photoassimilation of volatile fatty acids by purple non-sulfur bacteria: Experimental kinetics and dynamic modelling
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