[en] Today, scientific research in the field of natural textile dyeing is intensifying with the aim of contributing to the orientation of artisanal and industrial tinctorial practices, and to ensure the protection of the environment from harmful releases of effluents of synthetic dyes but also health of consumers. In this dynamic, physico-chemical, qualitative and quantitative, chromatographic and spectral analyses on the extract from fresh leaves of M. indica were carried out and allowed an informed application of this dye on cotton threads. With an extraction yield of about 9%, M. indica leaves have a total flavonoid content of 400.314±2.221mgEQ/g of dye powder. Hydrolyzable and condensed tanninsrateswere 10.157% and 44.850%, respectively. The HPTLC chromatographic profile of the dye extracted from leaves of M. indica indicated the presence of flavonols and xanthonoids (mangiferin). The vibrations of elongation νO-H, νC-N and that of deformation νNHrespectively of the alcohols, xanthonoids, amines and/or amides in infra-red suggest the presence of flavonic and xanthonoid colouring molecules that, through metal chelators and/or inter and/or intermolecular combinations, are responsible for the final colour shades obtained in tinctorial practice. High-performance liquid chromatography coupled with mass spectroscopy revealed the presence of major flavonoid molecules such as quercetin 3-O-hexose and kaempferol 3- O-hexose. Tinctorial practice techniques applied on cotton threads have allowed to have various shades of colors (champagne yellow, yellow corn, cow tail, vanilla, yellow March ...). In addition to being a natural acid dye, textile dye extracted from M. indica leaves can be classified as a metal dye in terms of the quality of shades obtained with the use of mordants such as alum, hydrated iron and copper sulfate.
