Investigation of MALDI-TOF Mass Spectrometry for Assessing the Molecular Diversity of Campylobacter jejuni and Comparison with MLST and cgMLST: A Luxembourg One-Health Study.
Feucherolles, Maureen; Nennig, Morgane; Becker, Sören Let al.
[en] There is a need for active molecular surveillance of human and veterinary Campylobacter infections. However, sequencing of all isolates is associated with high costs and a considerable workload. Thus, there is a need for a straightforward complementary tool to prioritize isolates to sequence. In this study, we proposed to investigate the ability of MALDI-TOF MS to pre-screen C. jejuni genetic diversity in comparison to MLST and cgMLST. A panel of 126 isolates, with 10 clonal complexes (CC), 21 sequence types (ST) and 42 different complex types (CT) determined by the SeqSphere+ cgMLST, were analysed by a MALDI Biotyper, resulting into one average spectra per isolate. Concordance and discriminating ability were evaluated based on protein profiles and different cut-offs. A random forest algorithm was trained to predict STs. With a 94% similarity cut-off, an AWC of 1.000, 0.933 and 0.851 was obtained for MLSTCC, MLSTST and cgMLST profile, respectively. The random forest classifier showed a sensitivity and specificity up to 97.5% to predict four different STs. Protein profiles allowed to predict C. jejuni CCs, STs and CTs at 100%, 93% and 85%, respectively. Machine learning and MALDI-TOF MS could be a fast and inexpensive complementary tool to give an early signal of recurrent C. jejuni on a routine basis.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Feucherolles, Maureen ; Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology, L-4422 Belvaux, Luxembourg
Nennig, Morgane; Epidemiology and Microbial Genomics, Laboratoire National de Santé, L-3555 Dudelange, Luxembourg
Becker, Sören L ; Institute of Medical Microbiology and Hygiene, Saarland University, 66421 Homburg, Germany ; Swiss Tropical and Public Health Institute, CH-4002 Basel, Switzerland ; University of Basel, CH-4003 Basel, Switzerland
Martiny, Delphine ; Université de Mons - UMONS ; National Reference Centre for Campylobacter, Centre Hospitalier Universitaire Saint-Pierre, Université Libre de Bruxelles (ULB), 1000 Brussels, Belgium
Losch, Serge; Laboratoire de Médecine Vétérinaire de l'Etat, L-3555 Dudelange, Luxembourg
Penny, Christian; Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology, L-4422 Belvaux, Luxembourg ; Cellule Scientifique, Chambre des Députés du Grand-Duché de Luxembourg, L-1728 Luxembourg, Luxembourg
Cauchie, Henry-Michel ; Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology, L-4422 Belvaux, Luxembourg
Ragimbeau, Catherine ; Epidemiology and Microbial Genomics, Laboratoire National de Santé, L-3555 Dudelange, Luxembourg
Language :
English
Title :
Investigation of MALDI-TOF Mass Spectrometry for Assessing the Molecular Diversity of Campylobacter jejuni and Comparison with MLST and cgMLST: A Luxembourg One-Health Study.
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