Isolation, purification, and metal-induced gelation of released polysaccharides from spent culture medium of Arthrospira

Cuellar-Bermudez, Sara P.; Bleus, Dries; Mees, Maarten A.; Struyf, Tom; Lama, Sanjaya; Vandamme, Dries; Adriaensens, Peter; Scherrers, Roger; Thielemans, Wim; Van den Ende, Wim; Wattiez, Ruddy; Muylaert, Koenraad

doi:10.1016/j.algal.2023.103010

Article (Scientific journals)

Isolation, purification, and metal-induced gelation of released polysaccharides from spent culture medium of Arthrospira

Cuellar-Bermudez, Sara P.; Bleus, Dries; Mees, Maarten A. et al.

2023 • In Algal Research, 70, p. 103010

Peer Reviewed verified by ORBi

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https://hdl.handle.net/20.500.12907/46267

DOI
10.1016/j.algal.2023.103010

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Keywords :

Extracellular polysaccharides; Gel rheology; Hydrogel; Limnospira; Spirulina; Ultrafiltration; Agronomy and Crop Science

Abstract :

[en] Cyanobacteria release polysaccharides that accumulate in the culture medium. In this study, released polysaccharides were isolated from the spent culture medium of an Arthrospira farm with a carbohydrate concentration of 136 mg L−1. The polysaccharides were concentrated 5.2 times by ultrafiltration using a 50 kDa polysulphone membrane and purified (desalting, protein removal). The polysaccharides were rich in uronic acid (159 mg g−1), had a low sulfate content (2.3 mg g−1), and contained 9 different monosaccharides, with 2-deoxy-D-galactose (27.9 %), fucose (22.7 %), glucose (22.0 %), and glucuronic acid (17.1 %) being dominant. A 1 % w/v polysaccharide concentrate formed stable gel beads in Al3+ and Fe3+ solutions. Rheological characterization of the gel revealed stable storage (G' = 38 Pa) and loss modulus (G" = 6 Pa) over a wide frequency range. This study demonstrates the feasibility to recover polysaccharides from Arthrospira spent culture medium and the potential to use these polysaccharides in food or biotechnological applications.

Disciplines :

Biochemistry, biophysics & molecular biology
Biotechnology
Microbiology

Author, co-author :

Cuellar-Bermudez, Sara P.; Laboratory of Aquatic Biology, KU Leuven, Kortrijk, Belgium ; Proteomic and Microbiology Department, University of Mons, Mons, Belgium

Bleus, Dries; Analytical and Circular Chemistry (ACC), Institute for Materials Research, Hasselt University, Hasselt, Belgium

Mees, Maarten A.; Sustainable Materials Lab, Department of Chemical Engineering, KU Leuven, Kortrijk, Belgium

Struyf, Tom; Lab of Molecular Plant Biology, Leuven, Belgium ; KU Leuven Plant Institute, Leuven, Belgium

Lama, Sanjaya; Analytical and Circular Chemistry (ACC), Institute for Materials Research, Hasselt University, Hasselt, Belgium

Vandamme, Dries; Analytical and Circular Chemistry (ACC), Institute for Materials Research, Hasselt University, Hasselt, Belgium

Adriaensens, Peter; Analytical and Circular Chemistry (ACC), Institute for Materials Research, Hasselt University, Hasselt, Belgium

Scherrers, Roger; Wyatt Technology Europe GmbH, Dernbach, Germany

Thielemans, Wim; Sustainable Materials Lab, Department of Chemical Engineering, KU Leuven, Kortrijk, Belgium

Van den Ende, Wim; Lab of Molecular Plant Biology, Leuven, Belgium ; KU Leuven Plant Institute, Leuven, Belgium

Wattiez, Ruddy ; Université de Mons - UMONS

Muylaert, Koenraad; Laboratory of Aquatic Biology, KU Leuven, Kortrijk, Belgium ; KU Leuven Plant Institute, Leuven, Belgium

Language :

English

Title :

Isolation, purification, and metal-induced gelation of released polysaccharides from spent culture medium of Arthrospira

Publication date :

March 2023

Journal title :

Algal Research

ISSN :

2211-9264

Publisher :

Elsevier B.V.

Volume :

Pages :

103010

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S2211926423000437?httpAccept=text/xml

Research unit :

Proteomics and Microbiology

Research institute :

R100 - Institut des Biosciences

Funders :

Interreg
Fonds Wetenschappelijk Onderzoek
Universiteit Hasselt

Funding text :

The authors want to acknowledge the financial support for this work from the EU Interreg France-Wallonie-Vlaanderen program through the ALPO project. The authors also acknowledge Prof. Dr. Erik Smolders of the Soil and Water Management Division at KU Leuven (Leuven) for the ICP analysis. Solid-state 13C CP/MAS spectroscopy has been executed by Gunther Reekmans at UHasselt. This work is supported by Hasselt University and the Research Foundation Flanders (Hercules project AUHL/15/2 - GOH3816N, Project G082121N).The authors want to acknowledge the financial support for this work from the EU Interreg France-Wallonie-Vlaanderen program through the ALPO project. The authors also acknowledge Prof. Dr. Erik Smolders of the Soil and Water Management Division at KU Leuven (Leuven) for the ICP analysis. Solid-state 13C CP/MAS spectroscopy has been executed by Gunther Reekmans at UHasselt. This work is supported by Hasselt University and the Research Foundation Flanders (Hercules project AUHL/15/2 - GOH3816N , Project G082121N ).

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