Asian hornet; Bumble bee; Butterfly; Commensalibacter; Functional genomics; Insect symbiont; Phylogenomics; Western honey bee; Veterinary (miscellaneous); Agricultural and Biological Sciences (miscellaneous); Animal Science and Zoology; Microbiology (medical); General Medicine
Abstract :
[en] [en] BACKGROUND: To understand mechanisms of adaptation and plasticity of pollinators and other insects a better understanding of diversity and function of their key symbionts is required. Commensalibacter is a genus of acetic acid bacterial symbionts in the gut of honey bees and other insect species, yet little information is available on the diversity and function of Commensalibacter bacteria. In the present study, whole-genome sequences of 12 Commensalibacter isolates from bumble bees, butterflies, Asian hornets and rowan berries were determined, and publicly available genome assemblies of 14 Commensalibacter strains were used in a phylogenomic and comparative genomic analysis.
RESULTS: The phylogenomic analysis revealed that the 26 Commensalibacter isolates represented four species, i.e. Commensalibacter intestini and three novel species for which we propose the names Commensalibacter melissae sp. nov., Commensalibacter communis sp. nov. and Commensalibacter papalotli sp. nov. Comparative genomic analysis revealed that the four Commensalibacter species had similar genetic pathways for central metabolism characterized by a complete tricarboxylic acid cycle and pentose phosphate pathway, but their genomes differed in size, G + C content, amino acid metabolism and carbohydrate-utilizing enzymes. The reduced genome size, the large number of species-specific gene clusters, and the small number of gene clusters shared between C. melissae and other Commensalibacter species suggested a unique evolutionary process in C. melissae, the Western honey bee symbiont.
CONCLUSION: The genus Commensalibacter is a widely distributed insect symbiont that consists of multiple species, each contributing in a species specific manner to the physiology of the holobiont host.
Disciplines :
Entomology & pest control Microbiology
Author, co-author :
Botero, Juliana; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000, Ghent, Belgium
Sombolestani, Atena Sadat; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000, Ghent, Belgium
Cnockaert, Margo; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000, Ghent, Belgium
Peeters, Charlotte; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000, Ghent, Belgium
Borremans, Wim; Research Group of Industrial Microbiology and Food Biotechnology, Department of Bioengineering Sciences, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium
De Vuyst, Luc; Research Group of Industrial Microbiology and Food Biotechnology, Department of Bioengineering Sciences, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium
Vereecken, Nicolas J; Agroecology Lab, Université libre de Bruxelles, Boulevard du Triomphe CP 264/02, 1050, Brussels, Belgium
Michez, Denis ; Université de Mons - UMONS > Faculté des Sciences > Service de Zoologie
Smagghe, Guy; Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium
Bonilla-Rosso, German; Department of Fundamental Microbiology, University of Lausanne, CH-1015, Lausanne, Switzerland
Engel, Philipp; Department of Fundamental Microbiology, University of Lausanne, CH-1015, Lausanne, Switzerland
Vandamme, Peter; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K. L. Ledeganckstraat 35, 9000, Ghent, Belgium. Peter.Vandamme@UGent.be
Language :
English
Title :
A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont.
FNRS/FWO joint program “EOS – Excellence Of Science Special Research Fund, Ghent University
Funding text :
We thank the Oxford Genomics Centre at the Welcome Centre for Human Genetics (funded by Wellcome Trust grant reference 203141/Z/16/Z) for the generation and initial processing of the sequencing data.This work was supported through funding of the FNRS/FWO joint program “EOS – Excellence Of Science” for the project “Climate change and its impact on pollination services” (CLiPS, no.3094785).
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