clean water; coastal biodiversity; coral reef fish; ecosystem protection; gregory; marine biodiversity; marine pollution; melanophores; melanosomes; reactive oxygen species; Stegastes apicalis; water contamination; water quality; Electronic, Optical and Magnetic Materials; Physics and Astronomy (miscellaneous); Atomic and Molecular Physics, and Optics; Pharmacology
Abstract :
[en] Reactive oxygen species (ROS) such as superoxide radicals O2−, hydroxyl radicals OH−, and hydrogen peroxide H2O2 may have detrimental effects on marine organisms, including their integuments and visual appearances. Although some studies have described the impact of ROS on marine ecosystems and species ecology, the influence on the optical response of the integuments of marine species and on their visual appearances remains unknown. In this article, we used histology and optical characterisation to show, for the first time, that skin melanophores (melanin-containing chromophores) of the coral reef fish, Stegastes apicalis, change their shapes and fluorescent proprieties upon oxidation with H2O2 radicals. Our observations also suggest that pheomelanosomes may occur in fish integuments, where, previously, it was thought that fish melanosomes only contain eumelanin. This investigation relied on light and electron microscopy and steady-state fluorimetry, as well as time-resolved streak imaging systems. We suggest that the changes in the morphological and spectral characteristics of melanophores can be used as a marker of physiological stress induced by environmental factors such as ROS. Moreover, S. apicalis may be used as a potential model for studying the interaction between the surrounding environment and natural organisms in biologically diverse ecosystems, such as the Great Barrier Reef in Australia.
Disciplines :
Physics
Author, co-author :
Mouchet, Sébastien R. ; School of Physics, University of Exeter, Exeter, United Kingdom ; Department of Physics, Namur Institute of Structured Matter (NISM), Institute of Life, Earth and Environment (ILEE), University of Namur, Namur, Belgium ; Queensland Brain Institute, The University of Queensland, Brisbane, Australia
Cortesi, Fabio ; Queensland Brain Institute, The University of Queensland, Brisbane, Australia ; School of the Environment, The University of Queensland, Brisbane, Australia
Bokic, Bojana ; Center for Photonics, Institute of Physics, University of Belgrade, Belgrade, Serbia
Lazovic, Vladimir ; Center for Photonics, Institute of Physics, University of Belgrade, Belgrade, Serbia
Vukusic, Pete ; School of Physics, University of Exeter, Exeter, United Kingdom
Marshall, N. Justin ; Queensland Brain Institute, The University of Queensland, Brisbane, Australia
Kolaric, Branko ; Université de Mons - UMONS > Faculté des Science > Service des Matériaux Micro et Nanophotoniques ; Center for Photonics, Institute of Physics, University of Belgrade, Belgrade, Serbia
Language :
English
Title :
Morphological and Optical Modification of Melanosomes in Fish Integuments upon Oxidation
Publication date :
December 2023
Journal title :
Optics
eISSN :
2673-3269
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI)
R400 - Institut de Recherche en Science et Ingénierie des Matériaux
Funders :
Belgian National Fund for Scientific Research BEWARE Fellowship Agathon De Potter Fund of the Royal Academy of Science, Letters and Fine Arts of Belgium Namur Institute of Structured Matter Australian Research Council EU: the EIC Pathfinder Challenges 2022 call through the Research Office of Naval Research Global Ministry of Science, Technological Development and Innovation of the Republic of Serbia FRS-FNRS Inter-university Attraction Pole: Photonics@be
Funding text :
S.R.M. was supported by the Belgian National Fund for Scientific Research (FRS-FNRS) (91400/1.B.309.18F) and a BEWARE Fellowship (Convention n°2110034) of the Walloon Region (COFUND Marie Skłodowska-Curie Actions of the European Union #847587) as a Postdoctoral Researcher. S.R.M. acknowledges a grant of the Agathon De Potter Fund of the Royal Academy of Science, Letters and Fine Arts of Belgium, a travel grant of FRS-FNRS, and a grant from the Namur Institute of Structured Matter (NISM). F.C. and N.J.M. were supported by the Australian Research Council (DE200100620 and FL140100197, respectively). BB and BK acknowledge the support of the of the EU: the EIC Pathfinder Challenges 2022 call through the Research Grant 101115149 (project ARTEMIS), the Office of Naval Research Global through the Research Grant N62902-22-1-2024 and the Ministry of Science, Technological Development and Innovation of the Republic of Serbia. B.K. also acknowledges financial support from FRS-FNRS; Inter-university Attraction Pole: Photonics@be (P7-35, Belgian Science Policy Office). The supporting bodies played no role in the design of the study, the collection of the samples, the analysis and interpretation of the data, and the writing of the manuscript.
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