[en] Natural selection can drive organisms to strikingly similar adaptive solutions, but the underlying molecular mechanisms often remain unknown. Several amphibians have independently evolved highly adhesive skin secretions (glues) that support a highly effective antipredator defence mechanism. Here we demonstrate that the glue of the Madagascan tomato frog, Dyscophus guineti, relies on two interacting proteins: a highly derived member of a widespread glycoprotein family and a galectin. Identification of homologous proteins in other amphibians reveals that these proteins attained a function in skin long before glues evolved. Yet, major elevations in their expression, besides structural changes in the glycoprotein (increasing its structural disorder and glycosylation), caused the independent rise of glues in at least two frog lineages. Besides providing a model for the chemical functioning of animal adhesive secretions, our findings highlight how recruiting ancient molecular templates may facilitate the recurrent evolution of functional innovations.
Disciplines :
Zoology
Author, co-author :
Zaman, Shabnam ; Ecology, Evolution & Genetics Research Group (bDIV), Biology Department, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium
Lengerer, Birgit ; Université de Mons - UMONS > Faculté des Sciences > Service de Biologie des Organismes Marins et Biomimétisme ; Evolutionary and Developmental Biology, Department of Zoology, University of Innsbruck, Technikerstr. 25, 6020, Innsbruck, Austria
Van Lindt, Joris; Center for Structural Biology, VIB-VUB and Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium
Saenen, Indra; Ecology, Evolution & Genetics Research Group (bDIV), Biology Department, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium
Russo, Giorgio; Center for Structural Biology, VIB-VUB and Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium
Bossaer, Laura ; Ecology, Evolution & Genetics Research Group (bDIV), Biology Department, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium
Tompa, Peter ; Center for Structural Biology, VIB-VUB and Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium ; Institute of Molecular Life Sciences, HUN-REN Research Centre for Natural Sciences, Magyar Tudósok Körútja 2, 1117, Budapest, Hungary
FLAMMANG, Patrick ; Université de Mons - UMONS > Faculté des Sciences > Service de Biologie des Organismes Marins et Biomimétisme
Roelants, Kim ; Ecology, Evolution & Genetics Research Group (bDIV), Biology Department, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium. Kim.Roelants@vub.be
Language :
English
Title :
Recurrent evolution of adhesive defence systems in amphibians by parallel shifts in gene expression.
S864 - Biologie des Organismes Marins et Biomimétisme
Research institute :
Biosciences
Funders :
Vrije Universiteit Brussel Austrian Science Fund
Funding text :
We thank Sunita Janssenswillen for assisting with protein extraction, Annelore Stroobants for assisting with gel electrophoresis, Leonard De Causmaecker for help with designing and performing the mechanical pull tests and Bram Vanschoenwinkel for help with statistics. This work is financed by FWO-Vlaanderen (grant no. G0D3214N) and Vrije Universiteit Brussel (grant no. SRP-30). S.Z., J.V.L. and G.R. are supported by doctoral fellowships from FWO-Vlaanderen (grants no. 11C6521N, 11D2520N and 1SC8222N, respectively). B.L. is supported by an ESPRIT grant of the Austrian Science Fund (FWF): [ESP 15]. P.F. is Research Director of the Fund for Scientific Research of Belgium (FRS-FNRS) and is supported by a FNRS PDR Grant no T.0088.20.
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