Design of femtosecond microstructured poly lactic acid temporal scaffolds coated with hydroxyapatite by pulse laser deposition method for bone tissue regeneration
Biopolymer cellular scaffolds; Bone tissue engineering; Femtosecond laser modification; Pulse laser deposition; Temporary cell matrices; Biopolymer cellular scaffold; Cell matrix; Cellular scaffolds; Deposition methods; Femtoseconds; Poly lactic acid; Temporary cell matrix; Electronic, Optical and Magnetic Materials; Atomic and Molecular Physics, and Optics; Electrical and Electronic Engineering
Abstract :
[en] The aim of the present study is to create porous poly lactic acid (PLA)-based temporal cellular scaffolds with specifically designed topographical orientation by means of femtosecond laser (fs)-induced microstructuring, additionally functionalized by a nanometric layer of hydroxyapatite (HA) by the pulse laser deposition (PLD) method. For this purpose, surface micromodification of PLA samples by means of a CPA Ti:sapphire fs laser system (τ = 150 fs, λ = 800 nm, ѵ = 0.5 kHz), operating at fluence F = 0.8 J/cm2 and scanning velocity V = 3.8 mm/s, was combined with PLD of thin layer of HA on the patterned PLA matrices for cellular scaffold surface additional nanofunctionalization. Each laser structured PLA scaffold was analyzed with respect to its control and laser processed surface, covered with HA. The multilevel structured scaffolds were investigated by SEM, EDX, 3D profilometer, AFM, micro-Raman and WCA analyses. Cytocompatibility studies with MG63 osteoblastic cells were also performed. Moreover, the cellular behavior was compared with the one observed on HA spin-coated fs microstructured PLA temporary scaffolds, in order to compare the two methods of functionalization. A disordered spreading on smooth surfaces to a tendency of cell orientation and elongation along the laser created grooves was monitored, along with increased alkaline phosphatase activity, which could essentially improve their subsequent practical application in engineering of personalized bone tissue.
Disciplines :
Chemistry
Author, co-author :
Angelova, L.; Institute of Electronics, Bulgarian Academy of Sciences, Sofia, Bulgaria
Daskalova, A.; Institute of Electronics, Bulgarian Academy of Sciences, Sofia, Bulgaria
Mincheva, Rosica ; Université de Mons - UMONS > Faculté des Sciences > Service des Matériaux Polymères et Composites
Filipov, E.; Institute of Electronics, Bulgarian Academy of Sciences, Sofia, Bulgaria
Dikovska, A.; Institute of Electronics, Bulgarian Academy of Sciences, Sofia, Bulgaria
Fernandes, M.H.; LAQV/REQUIMTE, University of Porto, Porto, Portugal ; Faculdade de Medicina Dentaria, Universidade do Porto, Porto, Portugal
Vig, S.; LAQV/REQUIMTE, University of Porto, Porto, Portugal ; Faculdade de Medicina Dentaria, Universidade do Porto, Porto, Portugal
Buchvarov, I.; Physics Department, Sofia University “St. Kliment Ohridski”, Sofia, Bulgaria
Language :
English
Title :
Design of femtosecond microstructured poly lactic acid temporal scaffolds coated with hydroxyapatite by pulse laser deposition method for bone tissue regeneration
BULGARIAN NATIONAL SCIENCE FUND EUROPEAN UNION’S H2020 research and innovation programme H2020 FET Open METAFAST
Funding text :
This research was funded by BULGARIAN NATIONAL SCIENCE FUND (NSF) under grant number No. KP-06-H48/6 (2020\u20132023), \u201CDevelopment of hybrid functional micro/nanoporous biomaterial scaffolds by ultra-fast laser modification\u201D, EUROPEAN UNION\u2019S H2020 research and innovation programme under the Marie Sklodowska-Curie Grant Agreement No. 861138, and H2020 FET Open METAFAST Grant Agreement No. 899673.
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