[en] In ART,1 optimized sperm preparation could separate spermatozoa from potentially harmful germs and cells, improving sperm parameters. This study compares the outcome of a routinely used two-layer density gradient with a three-layer protocol in terms of sperm and microbiological parameters. According to the results, a three-layer protocol was more effective.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Bekon, Affoua-Melissa; Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), 322, Rue Haute, 1000 Brussels, Belgium, Centre PMA, Dpt Gynecology/Obstetric, IVF laboratory, UHC Saint-Pierre, 322, Rue Haute, 1000 Brussels, Belgium, Faculté de Médecine et Pharmacie,Université de Mons (UMONS), Mons, Belgium
Martiny, Delphine ; Université de Mons - UMONS > Faculté de Médecine et de Pharmacie > Service du Doyen de la Faculté de Médecine et Pharmacie
Thomas, Anne-Laure; Centre PMA, Dpt Gynecology/Obstetric, IVF laboratory, UHC Saint-Pierre, 322, Rue Haute, 1000 Brussels, Belgium
Miendje, Yvette; Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), 322, Rue Haute, 1000 Brussels, Belgium
Debyttere, Anne-Laurence; Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), 322, Rue Haute, 1000 Brussels, Belgium
Autin, Candice; Centre PMA, Dpt Gynecology/Obstetric, IVF laboratory, UHC Saint-Pierre, 322, Rue Haute, 1000 Brussels, Belgium
Bertrand, Evelyne; Centre PMA, Dpt Gynecology/Obstetric, IVF laboratory, UHC Saint-Pierre, 322, Rue Haute, 1000 Brussels, Belgium. Electronic address: evelyne.bertrand@stpierre-bru.be
Language :
English
Title :
Optimization of the microbiological treatment of sperm for assisted reproductive technology (ART).
We want to thank the managers and all the technical and medical staff of the LHUB-ULB bacteriology department. We also want to thank the entire gynecology department of UHC Saint-Pierre, especially the managers and the staff of the IVF and andrology laboratories.
Boitrelle, F., Robin, G., Lefebvre, C., Bailly, M., Selva, J., Courcol, R., Lornage, J., Albert, M., Bacteriospermia in assisted reproductive techniques: effects of bacteria on spermatozoa and seminal plasma, diagnosis and treatment. Gynécologie Obstétrique Fertil. 40:4 (2012), 226–234, 10.1016/j.gyobfe.2012.01.003.
Borges, E.D., Berteli, T.S., Reis, T.F.D., Silva, A.S.D., Vireque, A.A., Microbial contamination in assisted reproductive technology: source, prevalence, and cost. J. Assist. Reprod. Genet. 37:1 (2020), 53–61.
Domes, T., Lo, K.C., Grober, E.D., Mullen, J.B., Mazzulli, T., Jarvi, K., The incidence and effect of bacteriospermia and elevated seminal leukocytes on semen parameters. Fertil. Steril. 97:5 (2012), 1050–1055, 10.1016/j.fertnstert.2012.01.124.
Ghasemian, F., Esmaeilnezhad, S., Moghaddam, M.J.M., Staphylococcus saprophyticus and Escherichia coli: tracking from sperm fertility potential to assisted reproductive outcomes. Clin. Exp. Reprod. Med., 48(2), 2021, 142.
Moretti, E., Capitani, S., Figura, N., Pammolli, A., Federico, M.G., Giannerini, V., Collodel, G., The presence of bacteria species in semen and sperm quality. J. Assist. Reprod. Genet. 26:1 (2009), 47–56, 10.1007/s10815-008-9283-5.
Štšepetova, J., Baranova, J., Simm, J., Parm, Ü., Rööp, T., Sokmann, S., Korrovits, P., Jaagura, M., Rosenstein, K., Salumets, A., Mändar, R., The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure. Reprod. Biol. Endocrinol. 18 (2020), 1–13.
World Health Organization, WHO Laboratory Manual for the Examination and Processing of Human Semen. 6th ed., 2021 Geneva https://apps.who.int/iris/handle/10665/343208 (accessed 7 February 2023).
