Antiradical and anti-acethylcholinesterase constituents from the methylene chloride extract of Ganoderma applanatum (Pers.) Pat (Ganodermataceae) and molecular docking study. - 2025
Antiradical and anti-acethylcholinesterase constituents from the methylene chloride extract of Ganoderma applanatum (Pers.) Pat (Ganodermataceae) and molecular docking study.
[en] Alzheimer's disease (AD) is a non-communicable disease with global impact. Inhibitors of acetylcholinesterase (AChE) are suitable therapies for AD. In this work, we report the isolation of antiacetylcholinesterase compounds from the methylene chloride (DCM) extract of the medical fungus Ganoderma applanatum (Pers.) Pat (Ganodermataceae). Chemical evaluation of this extract using chromatographic technics led to the isolation of a (1:1) mixture of ergosterol (1) and stellasterol (2), palmitic acid (3), ganodermanondiol (4), lucidumol B (5) and lupeol (6). Structures of these compounds were determined using spectroscopic analysis such as IR, MS, 1D & 2D NMR and literature. The acetylation reaction has been performed on the mixture (1 + 2) and compound 4, leading to the obtention the mixture of 3-acetyl-ergosterol (7) and 3-acetylstellasterol (8) along with 24-acetyl-ganodermanondiol (9) respectively. Total phenolic content was determined for DCM, Ethyl acetate and n-butanol extracts. To assess their antiradical scavenging potential, DPPH was used as free radical. The Inhibition power of acetylcholinesterase was evaluated in vitro using the Ellman reagent. Amongst all tested extracts, the DCM extract showed the high amount of total phenolic compounds with a value of 133.9512 mg EAG/g EX. The same extract showed a very good antiradical scavenging potential with an IC50 of 0.0021 mg/mL. The mixture (1 + 2) showed the highest antiradical scavenging activity with IC50 of 0.0770 mg/mL. The results obtained demonstrated that the acetylation has reduced the antiradical scavenging potential. Concerning the acetylcholinesterase inhibition power, the DCM extract and the mixture (1 + 2) showed a very good power with an inhibition percentage of 89%. The acetylation has also reduced the activity of the obtained derivative. The results provide insights into the potential efficacy of these compounds as acetylcholinesterase inhibitors. The binding interactions of the isolated and acetylated derivatives against acetylcholinesterase protein (PBP 3i6m) of Torpedo californica were studied using Autodock software. Ergosterol (-11.9 kcal/mol) binds better to the protein biding site through significant pi-sigma interactions.
Research center :
CMMI - Centre de Recherche en Microscopie et Imagerie Médicale
Disciplines :
Public health, health care sciences & services Neurology Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others Biochemistry, biophysics & molecular biology
Author, co-author :
Feunaing, Roméo Toko ; Department of Chemistry, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon
Gbaweng Yaya, Joël Abel ; Department of Chemistry, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon ; Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, Yaounde, Cameroon
Nyemb, Jean Noël ; Department of Refining and Petrochemistry, National Advanced School of Mines and Petroleum Industries, The University of Maroua, Kaele, Cameroon
Bassigue, Noël Issa; Department of Chemistry, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon
Ketsemen, Hervé Landry ; Department of Organic Chemistry, Faculty of Science, The University of Yaounde 1, Yaounde, Cameroon
HENOUMONT, Céline ; Université de Mons - UMONS > Faculté de Médecine et de Pharmacie > Service de Chimie générale, organique et biomédicale
Kandeda, Antoine Kavaye ; Department of Biology and Animal Physiology, Faculty of Science, The University of Yaounde 1, Yaounde, Cameroon
LAURENT, Sophie ; Université de Mons - UMONS > Faculté de Médecine et de Pharmacie > Service de Chimie générale, organique et biomédicale ; Department of Organic Chemistry, Faculty of Science, The University of Yaounde 1, Yaounde, Cameroon
Talla, Emmanuel; Department of Chemistry, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon
Language :
English
Title :
Antiradical and anti-acethylcholinesterase constituents from the methylene chloride extract of Ganoderma applanatum (Pers.) Pat (Ganodermataceae) and molecular docking study.
R550 - Institut des Sciences et Technologies de la Santé R100 - Institut des Biosciences
Funders :
International Foundation for Science
Funding text :
The authors are grateful to the International Foundation for Science (IFS) which partially supported this work through the grant number I1-F-6564-1 obtained by Dr. Yaya Gbaweng. Authors also thanks the bioprofiling platform supported by the European Regional Development Fund and the Walloon Region, Belgium.
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