Detection and quantifications; Electron paramagnetic resonance imaging; Electron paramagnetic resonance spectroscopy; Electron spins; High contrast; Performance; Physicochemical property; Property; Sensitive detection; Specific detection; Materials Science (all); Condensed Matter Physics; Surfaces and Interfaces; Spectroscopy; Electrochemistry
Abstract :
[en] Electron paramagnetic resonance (EPR) spectroscopy is a tool that provides sensitive detection of uncoupled electron spins for a variety of applications. This technique enables the specific detection and quantification of radical species while also being able of generating high-contrast, background-free images. However, the EPR labeling and imaging techniques encounter limitations mainly due to the instability of organic radicals from organic probes, which can influence the reliability and scope of the experiment. In that context, the use of nanodiamonds (NDs) in EPR may be a promising route for understanding their unique properties and potential biomedical applications. The ability to perform EPR imaging in combination with the stable intrinsic properties of paramagnetic centers within these particles raises the possibility of extending nanodiamond-based imaging capabilities. Herein, we present a preliminary demonstration of a practical spectroscopy and imaging application using nanosized diamond particles (<18 nm) for electron paramagnetic resonance imaging (EPRI). The discretization of two different nanodiamond production sources among the most studied NDs (HPHT or detonation) allows further characterization of their physicochemical properties. In addition, we have investigated variations in the physicochemical properties of nanodiamonds, including size effects and surface treatments. Finally, we provide experimental evidence of the conditions required for optimal spectroscopic and imaging resolution (R < 1 mm) as well as achievable EPR sensitivity.
Research center :
CMMI - Centre de Recherche en Microscopie et Imagerie Médicale
Disciplines :
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Garifo, Sarah ; Université de Mons - UMONS > Faculté de Médecine, Pharmacie et Sciences Biomédicales > Service de Chimie générale, organique et biomédicale
Stanicki, Dimitri ; Université de Mons - UMONS > Faculté de Médecine, Pharmacie et Sciences Biomédicales > Service de Chimie générale, organique et biomédicale
Vangijzegem, Thomas ; Université de Mons - UMONS > Faculté de Médecine, Pharmacie et Sciences Biomédicales > Service de Chimie générale, organique et biomédicale
Mellet, Philippe ; University of Bordeaux, CNRS, CRMSB, UMR 5536, Bordeaux F-33000, France ; INSERM, Bordeaux F-33000, France
Girard, Hugues A; Université Paris-Saclay, CEA, CNRS, NIMBE, Gif sur Yvette 91191, France
Arnault, Jean-Charles ; Université Paris-Saclay, CEA, CNRS, NIMBE, Gif sur Yvette 91191, France
Bégin-Colin, Sylvie ; UMR CNRS-UdS 7504, Institut de Physique et Chimie des Matériaux, CNRS, University of Strasbourg, Strasbourg 67034, France
Frapart, Yves-Michel; University of Paris Cité, CNRS, LCBPT, Paris F-75006, France
Muller, Robert ; Université de Mons - UMONS > Faculté de Médecine, Pharmacie et Sciences Biomédicales > Service du Doyen de la Faculté de Médecine, Pharmacie et Sciences Biomédicales ; Center for Microscopy and Molecular Imaging (CMMI), Gosselies B-6041, Belgium
Laurent, Sophie ; Université de Mons - UMONS > Faculté de Médecine et de Pharmacie > Service de Chimie générale, organique et biomédicale ; Center for Microscopy and Molecular Imaging (CMMI), Gosselies B-6041, Belgium
Language :
English
Title :
Tailoring Nanodiamonds for High-Contrast EPR Imaging: Size, Surface Properties, and Spectroscopic Performance.
R550 - Institut des Sciences et Technologies de la Santé R100 - Institut des Biosciences
Funders :
Centre National de la Recherche Scientifique Waalse Gewest ARC Programs of the French Community of Belgium
Funding text :
The authors acknowledge P. Massot from CNRS-UMR5536 and R. Abregel from MAP5-UMR 8145 for their invaluable assistance throughout the project. The authors thank the laboratories of J.-M. Raquez for providing access to TGA equipment, P. Duez for the access to ICP-AES instrument, and the Center for Microscopy and Molecular Imaging (CMMI, supported by the European Regional Development Fund and the Walloon Region). The authors are also grateful to Materia Nova for the collaboration and support in the XPS characterizations. This work was supported by the Fond National de la Recherche Scientifique (FNRS), the ARC Programs of the French Community of Belgium, and the Walloon Region (Prother-Wal and Interreg projects).
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