Multigenerational exposure of microplastics on the microbiota of E. affinis (copepod): a comparative study between biodegradable and nonbiodegradable microplastics
[en] The accumulation of plastic debris around the world, especially in marine environments, has been well documented during the past decades. Recent studies have found that inorganic surfaces of microplastics (MPs) can be used by microorganisms as living substrates and form an ecosystem named “plastisphere.” Some microorganisms present in MPs are capable of producing polymer-degrading enzymes. In addition, MPs can also serve as vectors and carry microorganisms (including potential pathogens) into higher trophic levels through their ingestion by animals. In this study, impacts on copepod microbiota during chronic exposure to MPs were investigated by exposing copepods to a classic single-use polymer (low-density polyethylene (LDPE)) and a biodegradable polymer (polybutylene adipate terephthalate (PBAT)). Copepods were exposed to “virgin” and “weathered” MPs during four generations at an environmentally relevant concentration of 300 µg/L, followed by one “detoxification” generation without MP exposition. Impacts of MP exposure on copepod microbiota were investigated using 16S rRNA gene high-throughput sequencing. The result of nonmetric multidimensional scaling (NMDS) analysis showed that copepods (with or without MP exposure) carried distinguishable microbiota as compared with the microbiota of water and microalgae used for maintaining copepods. According to the results of permutational analysis of variance (PERMANOVA), the microbiota of MP-exposed (both PBAT and LDPE) copepods was significantly different from the microbiota of unexposed copepods during generations one to four. After “detoxification,” however, no significant difference in microbiota composition was observed among all generation five copepods. Altogether, impacts on copepod microbiota of MP exposure for multiple generations were observed, despite plastic origin (biodegradable or not) and aging conditions. Furthermore, copepod microbiota seemed to return to their original structure as soon as the MP exposure stopped.
Disciplines :
Materials science & engineering
Author, co-author :
Thery, Jérémy; Univ. Littoral Côte d’Opale, CNRS, Univ. Lille, IRD, UMR 8187, LOG, Laboratoire d’Océanologie et de Géosciences, Wimereux, France ; Centre d’Innovation et de Recherche des Matériaux et Polymères (CIRMAP), Service des Matériaux Polymères et Composites (SMPC), Université de Mons, Mons, Belgium
Li, Luen-Luen; Univ. Littoral Côte d’Opale, CNRS, Univ. Lille, IRD, UMR 8187, LOG, Laboratoire d’Océanologie et de Géosciences, Wimereux, France
Das, Shagnika; Univ. Littoral Côte d’Opale, CNRS, Univ. Lille, IRD, UMR 8187, LOG, Laboratoire d’Océanologie et de Géosciences, Wimereux, France ; Center for Marine Science and Technology, Amity Institute of Biotechnology, Amity University, Noida, India
Dufour, Dylan; Univ. Littoral Côte d’Opale, CNRS, Univ. Lille, IRD, UMR 8187, LOG, Laboratoire d’Océanologie et de Géosciences, Wimereux, France
Benali, Samira ; Université de Mons - UMONS > Faculté des Science > Service des Matériaux Polymères et Composites
Raquez, Jean-Marie ; Université de Mons - UMONS > Faculté des Science > Service des Matériaux Polymères et Composites
Souissi, Sami; Univ. Littoral Côte d’Opale, CNRS, Univ. Lille, IRD, UMR 8187, LOG, Laboratoire d’Océanologie et de Géosciences, Wimereux, France
Monchy, Sébastien; Univ. Littoral Côte d’Opale, CNRS, Univ. Lille, IRD, UMR 8187, LOG, Laboratoire d’Océanologie et de Géosciences, Wimereux, France
Language :
English
Title :
Multigenerational exposure of microplastics on the microbiota of E. affinis (copepod): a comparative study between biodegradable and nonbiodegradable microplastics
R400 - Institut de Recherche en Science et Ingénierie des Matériaux
Funding text :
This study is a part of the Ph.D. thesis of JT from the University of Mons and the University of Littoral Côte d’Opale, funded in equal parts by the two universities. This work has been financially supported by the French State and the French Region Hauts-de-France, in the framework of the project CPER IDEAL 2021-2029. Samira Benali acknowledge supports by the European Community (FEDER) for general support in the frame of LCFM-BIOMAT. Jean-Marie Raquez is a FRS-FNRS Research Associate. AcknowledgmentsThis study is a part of the Ph.D. thesis of JT from the University of Mons and the University of Littoral Côte d’Opale, funded in equal parts by the two universities. This work has been financially supported by the French State and the French Region Hauts-de-France, in the framework of the project CPER IDEAL 2021-2029. Samira Benali acknowledge supports by the European Community (FEDER) for general support in the frame of LCFM-BIOMAT. Jean-Marie Raquez is a FRS-FNRS Research Associate.
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