[en] AIMS: Diets and parasites influence the gut bacterial symbionts of bumble bees, but potential interactive effects remain overlooked. The main objective of this study was to assess the isolated and interactive effects of sunflower pollen, its phenolamides, and the widespread trypanosomatid Crithidia sp. on the gut bacterial symbionts of Bombus terrestris males.
METHODS AND RESULTS: Bumble bee males emerged in microcolonies fed on either (i) willow pollen (control), (ii) sunflower pollen, or (iii) willow pollen spiked with phenolamide extracts from sunflower pollen. These microcolonies were infected by Crithidia sp. or were pathogen-free. Using 16S rRNA amplicon sequencing (V3-V4 region), we observed a significant alteration of the beta diversity but not of the alpha diversity in the gut microbial communities of males fed on sunflower pollen compared to males fed on control pollen. Similarly, infection by the gut parasite Crithidia sp. altered the beta diversity but not the alpha diversity in the gut microbial communities of males, irrespective of the diet. By contrast, we did not observe any significant alteration of the beta or alpha diversity in the gut microbial communities of males fed on phenolamide-enriched pollen compared to males fed on control pollen. Changes in the beta diversity indicate significant dissimilarities of the bacterial taxa between the treatment groups, while the lack of difference in alpha diversity demonstrates no significant changes within each treatment group.
CONCLUSIONS: Bumble bees harbour consistent gut microbiota worldwide, but our results suggest that the gut bacterial communities of bumble bees are somewhat shaped by their diets and gut parasites as well as by the interaction of these two factors. This study confirms that bumble bees are suitable biological surrogates to assess the effect of diet and parasite infections on gut microbial communities.
Disciplines :
Microbiology
Author, co-author :
GEKIERE, Antoine ; Université de Mons - UMONS > Faculté des Science > Service de Zoologie
Vanderplanck, Maryse ; Université de Mons - UMONS > Faculté des Science > Service de Zoologie ; CEFE, CNRS, Univ Montpellier, EPHE, IRD, Montpellier, France, 1919 Route de Mende, 34293 Montpellier, France
Hettiarachchi, Amanda; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, 35 K.L. Ledeganckstraat, 9000 Ghent, Belgium
Semay, Irène ; Université de Mons - UMONS > Faculté des Science > Service de Synthèse et spectrométrie de masse organiques
Gerbaux, Pascal ; Université de Mons - UMONS > Faculté des Science > Service de Synthèse et spectrométrie de masse organiques
Michez, Denis ; Université de Mons - UMONS > Faculté des Science > Service de Zoologie
Joossens, Marie; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, 35 K.L. Ledeganckstraat, 9000 Ghent, Belgium
Vandamme, Peter; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, 35 K.L. Ledeganckstraat, 9000 Ghent, Belgium
Language :
English
Title :
A case study of the diet-microbiota-parasite interplay in bumble bees
Fonds De La Recherche Scientifique - FNRS Research Foundation Flanders
Funding text :
This work was a part of the ARC ‘Actions de Recherche Concertées’ project ‘METAFLORE, 2019–2023’. This work was also partly supported by the ‘Fonds de la Recherche Scientifique—FNRS’ and the Research Foundation-Flanders (FWO) under EOS Project CLIPS (n°3 094785). A.G. is supported by a F.R.S.-FNRS PhD grant ‘Aspirant’. The PhD grant of I.S. was supported by the ARC project METAFLORE.
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