[en] Fertilization is a highly coordinated process that relies on intricate protein interactions. Among these, Cysteine-Rich Secretory Proteins (CRISPs) play a key role, with three members identified in humans. While CRISP1 and CRISP3 associate with the sperm surface during epididymal transit, CRISP2 is expressed during spermatogenesis. Its functional role appears to be mediated by interactions with (i) Sertoli cells, (ii) ion channels such as CatSper and RyR, and (iii) a yet unidentified receptor on the oolemma. In this study, using the yeast two-hybrid (Y2H) technique, we aim to identify the hCRISP2 interactome to gain mechanistic insights into its contribution to sperm physiology and fertilization.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Masai, Thibault ; Université de Mons - UMONS > Faculté des Sciences > Service de Biologie cellulaire
Tafforeau, Lionel ; Université de Mons - UMONS > Faculté des Sciences > Service de Biologie cellulaire
Lybaert, Pascale; ULB - Université Libre de Bruxelles > Research laboratory on Human Reproduction
Hennebert, Elise ; Université de Mons - UMONS > Faculté des Sciences > Service de Biologie cellulaire
Language :
English
Title :
Towards the Identification of hCRISP2 Interactome
Alternative titles :
[fr] Vers l'identification de l'interactive de CRISP2 humaine
Original title :
[en] Towards the Identification of hCRISP2 Interactome
Publication date :
14 April 2025
Event name :
23rd European Testis Workshop (ETW)
Event organizer :
Katja Teerds Tzviya Zeev Ben Mordehai Bart Gadella Ans van Pelt Callista Mulder Geert Hamer Willy Baarends
Event place :
St Michielsgestel, Netherlands
Event date :
From 13 April 2025 to 17 April 2025
Audience :
International
Peer review/Selection committee :
Peer reviewed
Research unit :
S815 - Biologie cellulaire
Research institute :
R100 - Institut des Biosciences R550 - Institut des Sciences et Technologies de la Santé
