A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria-A Pilot Study.
{"lastName":"Laumen", "firstNames":"Jolein Gyonne Elise","affiliations":["STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium","Laboratory of Medical Microbiology, University of Antwerp, 2610 Wilrijk, Belgium"],"ids":["SC:57205701202""OR:0000-0003-0168-5622"]}; {"lastName":"Abdellati", "firstNames":"Saïd","affiliations":["Clinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"],"ids":["SC:22946293200""OR:0000-0002-9620-8257"]}; {"lastName":"Van Dijck", "firstNames":"Christophe","affiliations":["STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium","Laboratory of Medical Microbiology, University of Antwerp, 2610 Wilrijk, Belgium"],"ids":["SC:6507970927""OR:0000-0001-8010-1351"]}et al.
Neisseria species; antimicrobial susceptibility; surveillance; Microbiology; Biochemistry; Pharmacology, Toxicology and Pharmaceutics (all); Microbiology (medical); Infectious Diseases; Pharmacology (medical); General Pharmacology, Toxicology and Pharmaceutics
Abstract :
[en] Commensal Neisseria provide a reservoir of resistance genes that can be transferred to the pathogens Neisseria gonorrhoeae and N. meningitidis in the human oropharynx. Surveillance programs are thus needed to monitor resistance in oropharyngeal commensal Neisseria, but currently the isolation and antimicrobial susceptibility testing of these commensals is laborious, complex and expensive. In addition, the posterior oropharyngeal/tonsillar swab, which is commonly used to sample oropharyngeal Neisseria, is poorly tolerated by many individuals. We evaluated an alternative non-invasive method to isolate oropharyngeal commensal Neisseria and to detect decreased susceptibility to azithromycin using selective media (LBVT.SNR) with and without azithromycin (2 µg/mL). In this pilot study, we compared paired posterior oropharyngeal/tonsillar swabs and oral rinse-and-gargle samples from 10 participants and demonstrated that a similar Neisseria species diversity and number of colonies were isolated from both sample types. Moreover, the proportion of Neisseria colonies that had a decreased susceptibility to azithromycin was similar in the rinse samples compared to the swabs. This pilot study has produced encouraging data that a simple protocol of oral rinse-and-gargle and culture on plates selective for commensal Neisseria with and without a target antimicrobial can be used as a surveillance tool to monitor antimicrobial susceptibility in commensal oropharyngeal Neisseria. Larger studies are required to validate these findings.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
{"lastName":"Laumen", "firstNames":"Jolein Gyonne Elise","affiliations":["STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium","Laboratory of Medical Microbiology, University of Antwerp, 2610 Wilrijk, Belgium"],"ids":["SC:57205701202""OR:0000-0003-0168-5622"]}
{"lastName":"Abdellati", "firstNames":"Saïd","affiliations":["Clinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"],"ids":["SC:22946293200""OR:0000-0002-9620-8257"]}
{"lastName":"Van Dijck", "firstNames":"Christophe","affiliations":["STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium","Laboratory of Medical Microbiology, University of Antwerp, 2610 Wilrijk, Belgium"],"ids":["SC:6507970927""OR:0000-0001-8010-1351"]}
Martiny, Delphine ; Université de Mons - UMONS ; Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), Université Libre de Bruxelles, 1000 Brussels, Belgium
{"lastName":"De Baetselier", "firstNames":"Irith","affiliations":["Clinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"],"ids":["SC:56444451900""OR:0000-0002-1804-252X"]}
{"lastName":"Manoharan-Basil", "firstNames":"Sheeba Santhini","affiliations":["STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"],"ids":["SC:57191344249""OR:0000-0001-8421-5137"]}
Van den Bossche, Dorien; Clinical and Reference Laboratory, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium
{"lastName":"Kenyon", "firstNames":"Chris","affiliations":["STI Unit, Department of Clinical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium","Division of Infectious Diseases and HIV Medicine, University of Cape Town, Cape Town 7700, South Africa"],"ids":["SC:16417556900""OR:0000-0002-2557-8998"]}
Language :
English
Title :
A Novel Method to Assess Antimicrobial Susceptibility in Commensal Oropharyngeal Neisseria-A Pilot Study.
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