Insects; PCR; combination of methods; feed; legislation; light microscopy; mass spectrometry; metagenomics; processed animal proteins; vibrational spectroscopy; Proteins; Animals; Insecta; Microscopy/methods; Polymerase Chain Reaction; Proteins/analysis; Animal Feed/analysis; Analytical method; Animal feed; Combination of method; European legislation; Insect; Nutritional qualities; Microscopy; Food Science; Chemistry (all); Toxicology; Public Health, Environmental and Occupational Health; Health, Toxicology and Mutagenesis; General Chemistry; General Medicine
Abstract :
[en] Since their approval for use in aquaculture in 2017, processed insect proteins have been extensively studied for their nutritional quality in animal feed. This new type of meal is highly promising but requires, as for other products used in animal feed, strict sanitary control in accordance with European legislation. Within this legal framework, light microscopy and PCR remain the official methods but have some analytical limitations that other methods could overcome. This paper aims to provide an overview of the European legislation concerning use of processed insect proteins, but also to highlight the advantages and disadvantages of the official methods for their analysis. It also points out other analytical methods, which have already proved their worth for the analysis of processed animal proteins, which could be used as complementary methods.
Disciplines :
Agriculture & agronomy Entomology & pest control
Author, co-author :
Anselmo, Abigaël ; Université de Mons - UMONS > Faculté des Science > Service de Zoologie
Veys, Pascal; Walloon Agricultural Research Centre (CRA-W), Quality and Authentication of Products Unit (QAF), Gembloux, Belgium
Fumière, Olivier; Walloon Agricultural Research Centre (CRA-W), Quality and Authentication of Products Unit (QAF), Gembloux, Belgium
Lecrenier, Marie-Caroline; Walloon Agricultural Research Centre (CRA-W), Quality and Authentication of Products Unit (QAF), Gembloux, Belgium
Cordonnier, Alexandra; Walloon Agricultural Research Centre (CRA-W), Quality and Authentication of Products Unit (QAF), Gembloux, Belgium
Michez, Denis ; Université de Mons - UMONS > Faculté des Sciences > Service de Zoologie
Baeten, Vincent; Walloon Agricultural Research Centre (CRA-W), Quality and Authentication of Products Unit (QAF), Gembloux, Belgium
Language :
English
Title :
Challenges related to the application of analytical methods to control insect meals in the context of European legislation
Publication date :
June 2023
Journal title :
Food Additives and Contaminants. Part A. Chemistry, Analysis, Control, Exposure and Risk Assessment
Abo Arab RB, El-Tawelah NM, Abouelatta AM, Hamza AM. 2022. Potential of selected plant essential oils in management of Sitophilus oryzae (L.) and Rhiyzopertha dominica (F.) on wheat grains. Bull Natl Res Cent. 46 (1): 192. doi: 10.1186/s42269-022-00894-x
Akinbuluma MD, Okunlola OT, Alabi OY, Omobusuyi DO. 2022. Towards food security: essential oil components as protectants against the rice weevil. Sitophilus Oryzae. 15 (2): 5. doi: 10.54319/jjbs/150205
Anselmo A. 2022. Detection of processed animal proteins (PAPs) by near-infrared microscopy (NIRM). Presented at: vibrational spectroscopy and Chemometrics workshop. 10th International Symposium of Recent Advances in Food Analysis; Sep 6–9; Prague, Czech Republic.
Anselmo A, Cordonnier A, Veys P, Stevens F, Fernández Pierna JA, Baeten V. 2022. Detection of insect meal in animal feed by the use of near-infrared microscopy (NIRM). Poster Session Presented at: 10th International Symposium of Recent Advances in Food Analysis; Sep 6-9; Prague, Czech Republic.
Baeten V, Dardenne P. 2016. NIR-BASED detection of contaminants in food and feed. Feedipedia. [accessed 2021 Jan 13]. https://www.feedipedia.org/content/nir-based-detection-contaminants-food-and-feed.
Baeten V, Fernández Pierna JA, Vermeulen P, Dardenne P. 2010. NIR hyperspectral imaging methods for quality and safety control of food and feed products: contributions to four European Projects. NIR News. 21 (6): 10–13. doi: 10.1255/nirn.1200
Baeten V, von Holst C, Garrido A, Vancutsem J, Michotte Renier A, Dardenne P. 2005. Detection of banned meat and bone meal in feedstuffs by near-infrared microscopic analysis of the dense sediment fraction. Anal Bioanal Chem. 382 (1): 149–157. doi: 10.1007/s00216-005-3193-5
Baeten V, Vermeulen P, Vancutsem J, Bosch J, Berben G, Brambilla G, Boix A, Portetelle D, Garrido A, Marin DP, et al. 2004. Comparison and Complementarity of the methods. In: Strategies and Methods to detect and quantify mammalian tissues in feedingstuffs. Bruxelles - Belgium: European Commission. p. 12. http://STRATFEED.cra.wallonie.be.
Baeten V, Von Holst C, Banks I, Michotte Renier A, Murray I, Dardenne P. 2004. The near-infrared microscopic (NIRM) method : combination of the advantages of optical microscopy and near-infrared spectroscopy (WP5-NIRM). In: Strategies and Methods to detect and quantify mammalian tissues in feedingstuffs. Bruxelles - Belgium: European Commission. http://STRATFEED.cra.wallonie.be.
Barragan-Fonseca KB, Dicke M, van Loon JJA. 2017. Nutritional value of the black soldier fly (Hermetia illucens L.) and its suitability as animal feed–a review. J Insects Food Feed. 3 (2): 105–120. doi: 10.3920/JIFF2016.0055
Belghit I, Lock E-J, Fumière O, Lecrenier M-C, Renard P, Dieu M, Berntssen M, Palmblad M, Rasinger J. 2019. Species-specific discrimination of insect meals for aquafeeds by direct comparison of Tandem mass spectra. Animals. 9 (5): 222. doi: 10.3390/ani9050222
Belghit I, Varunjikar M, Lecrenier M-C, Steinhilber A, Niedzwiecka A, Wang YV, Dieu M, Azzollini D, Lie K, Lock E-J, et al. 2021. Future feed control–Tracing banned bovine material in insect meal. Food Control. 128: 108183. doi: 10.1016/j.foodcont.2021.108183
Boix A, Fernández Pierna JA, von Holst C, Baeten V. 2012. Validation of a near infrared microscopy method for the detection of animal products in feedingstuffs: results of a collaborative study. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 29 (12): 1872–1880. doi: 10.1080/19440049.2012.712551
Bonjour EL, Opit GP, Hardin J, Jones CL, Payton ME, Beeby RL. 2011. Efficacy of ozone fumigation against the major grain pests in stored wheat. J ECon entomol. 104 (1): 308–316. doi: 10.1603/EC10200
Boopathy B, Rajan A, Radhakrishnan M. 2022. Ozone: an alternative fumigant in controlling the stored product insects and pests: a status report. Ozone: science & Engineering. 44 (1): 79–95. doi: 10.1080/01919512.2021.1933899
Bousquet Y. 1990. Beetles associated with stored products in Canada: an identification guide. Research Branch. Ottawa, Ontario: Agriculture Canada.
Chaudhari AK, Singh VK, Kedia A, Das S, Dubey NK. 2021. Essential oils and their bioactive compounds as eco-friendly novel green pesticides for management of storage insect pests: prospects and retrospects. Environ Sci Pollut Res Int. 28 (15): 18918–18940. doi: 10.1007/s11356-021-12841-w
Daniso E, Melpignano P, Tulli F. 2020. An OLED-based genosensor for the detection of Hermetia illucens in feeds. Food Control. 113: 107179. doi: 10.1016/j.foodcont.2020.107179
Dardenne P, Baeten V, Berben G, Vermeulen P, Garrido Varo A, van Raamsdonk L, Brambilla G, Murray I, von Holst C. 2005. Strategies and methods to detect and quantify mammalian tissues in feedingstuffs. Dardenne, P. Bruxelles: European Commission.
De Marco M, Martínez S, Hernandez F, Madrid J, Gai F, Rotolo L, Belforti M, Bergero D, Katz H, Dabbou S, et al. 2015. Nutritional value of two insect larval meals (Tenebrio molitor and Hermetia illucens) for broiler chickens: apparent nutrient digestibility, apparent ileal amino acid digestibility and apparent metabolizable energy. Anim Feed Sci Technol. 209: 211–218. doi: 10.1016/j.anifeedsci.2015.08.006
Debode F, Marien A, Gérard A, Francis F, Fumière P, Berben G. 2017. Development of real-time PCR tests for the detection of Tenebrio molitor in food and feed. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 34 (8): 1421–1426. doi: 10.1080/19440049.2017.1320811
Delarozadelgado B, Soldado A, Martinez-Fernandez A, Vicente F, Garridovaro A, Perezmarin D, Delahaba M, Guerreroginel J. 2007. Application of near-infrared microscopy (NIRM) for the detection of meat and bone meals in animal feeds: a tool for food and feed safety. Food Chem. 105 (3): 1164–1170. doi: 10.1016/j.foodchem.2007.02.041
Dobrovolny S, Blaschitz M, Weinmaier T, Pechatschek J, Cichna-Markl M, Indra A, Hufnagl P, Hochegger R. 2019. Development of a DNA metabarcoding method for the identification of fifteen mammalian and six poultry species in food. Food Chem. 272: 354–361. doi: 10.1016/j.foodchem.2018.08.032
EURL-AP 2013. EURL-AP Standard Operating Procedure - Slide preparation and mounting. https://www.eurl.craw.eu/wp-content/uploads/2021/01/EURL-AP-SOP-slide-mounting-V1.0.pdf.
EURL-AP 2014. EURL-AP Standard Operating Procedure - DNA extraction using the ‘Wizard® Magnetic DNA purification system for Food’ kit. https://www.eurl.craw.eu/wp-content/uploads/2021/01/EURL-AP-SOP-DNA-extraction-V1.1.pdf.
EURL-AP 2021a. EURL-AP Standard Operating Procedure - Detection of ruminant DNA in feed using real-time PCR. https://www.eurl.craw.eu/wp-content/uploads/2021/05/EURL-AP-SOP-Ruminant-PCR-V1.3.pdf.
EURL-AP 2021b. EURL-AP Standard Operating Procedure - Detection of pig DNA in feed using real-time PCR. https://www.eurl.craw.eu/wp-content/uploads/2021/09/EURL-AP-SOP-Pig-PCR-V1.0.pdf.
EURL-AP 2022a. EURL-AP Standard Operating Procedure - On the use of the observation flowchart for light microscopy. https://www.eurl.craw.eu/wp-content/uploads/2020/12/EURL-AP-SOP-observation-flowchart-V1.0.pdf.
EURL-AP 2022b. EURL-AP Standard Operating Procedure - Detection of poultry (chicken and turkey) DNA in feed using real-time PCR. https://www.eurl.craw.eu/wp-content/uploads/2021/09/EURL-AP-SOP-Poultry-PCR-V1.0.pdf.
European Commission. 1994. 94/381/EC : Commission Decision of 27 June 1994 concerning certain protection measures with regard to bovine spongiform encephalopathy and the feeding of mammalian derived protein (Text with EEA relevance). Official Journal of the European Union. L. 172: 23–24.
European Commission. 2001. Regulation (EC) No 999/2001 of the European Parliament and the Council of 22 May 2001 laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies. O JE U. L147: 1–40.
European Commission. 2002. Regulation (EC) No 1774/2002 of the European Parliament and of the Council of 3 October 2002. O J EU. L 273/. 1: 95.
European Commission. 2003. Commission directive 2003/123/EC of 23 December 2003 on the analytical method for the determination of constituents of animal origin for the official control of feedingstuffs. OJEU. L339/78: 7.
European Commission. 2009. Commission regulation (EC) No 125/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed. OJEU. L54/1: 1–130.
European Commission. 2013. Commission Regulation (EU) No 56/2013 of 16 January 2013 amending Annexes I and IV to Regulation (EC) No 999/2001 of the European Parliament and of the Council laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies.OJEU. L21/3: 14.
European Commission. 2017. Règlement (UE) 2017/893 de la Commission - du 24 mai 2017 - modifiant les annexes I et IV du règlement (CE) No 999/2001 du Parlement européen et du Conseil et les annexes X, XIV et XV du règlement (UE) No 142/2011 de la Commission concernant les dispositions relatives aux protéines animales transformées. O J E U. L138: 92–116.
European Commission. 2021a. Commission Regulation (EU) 2021/1372 of 17 August 2021 amending Annex IV to Regulation (EC) No 999/2001 of the European Parliament and of the Council as regards the prohibition to feed non-ruminant farmed animals, other than fur animals, with protein derived from animals. Off. 64 (L295): 1–21.
European Commission. 2021b. Commission Regulation (EU) 2021/1925 of 5 November 2021 amending certain Annexes to Regulation (EU) No 142/2011 as regards the requirements for placing on the market of certain insect products and the adaptation of a containment method. OJEU. L393: 4–8.
European Commission. 2022. Commission Implementing Regulation (EU) 2022/893 of 7 June 2022 amending Annex VI to Regulation (EC) No 152/2009 as regards the methods of analysis for the detection of constituents of terrestrial invertebrates for the official control of feed.OJEU. / 24: 12.
FAO (Food and Agriculture Organization of the United Nations). 2013. Edible insects: future prospects for food and feed security. FAO Forestry Paper No. 171.
Fernández Pierna J. A, Baeten V, Renier AM, Cogdill RP, Dardenne P. 2004. Combination of support vector machines (SVM) and near-infrared (NIR) imaging spectroscopy for the detection of meat and bone meal (MBM) in compound feeds. J Chemometrics. 18 (7-8): 341–349. doi: 10.1002/cem.877
Fernández Pierna JA, Dardenne P, Baeten V. 2010. In-house validation of a near Infrared Hyperspectral Imaging Method for detecting processed animal proteins in compound Feed. J Near Infrared Spectrosc. 18 (2): 121–133. doi: 10.1255/jnirs.872
Fumière O, Dubois M, Baeten V, von Holst C, Berben G. 2006. Effective PCR detection of animal species in highly processed animal by-products and compound feeds. Anal Bioanal Chem. 385 (6): 1045–1054. doi: 10.1007/s00216-006-0533-z
Fumière O, Veys P, Boix A, von Holst C, Baeten V, Berben G. 2009. Methods of detection, species identification and quantification of processed animal proteins in feedingstruffs. Biotechnol Agron Soc Environ. 13: 59–70. https://popups.uliege.be/1780-4507/index.php?id=3525.
Fumière O, Zagon J, Lecrenier M-C. 2022. Re-authorization of gelatin and collagen of ruminant origin in non-ruminant feed: a new analytical challenge for the control of the feed ban. ? Biotechnol Agron Soc Environ. 26: 303–308. doi: 10.25518/1780-4507.20059
Garrido-Sanz L, Àngel Senar M, Piñol J. 2022. Drastic reduction of false positive species in samples of insects by intersecting the default output of two popular metagenomic classifiers. Kalendar R, editor. PLOS One. 17 (10): e0275790. doi: 10.1371/journal.pone.0275790
Hellberg RS, Hernandez BC, Hernandez EL. 2017. Identification of meat and poultry species in food products using DNA barcoding. Food Control. 80: 23–28. doi: 10.1016/j.foodcont.2017.04.025
Hong J, Han T, Kim YY. 2020. Mealworm (Tenebrio molitor Larvae) as an alternative protein source for monogastric animal: a review. Animals. 10 (11): 2068. doi: 10.3390/ani10112068
Kröncke N, Benning R. 2022. Determination of moisture and protein content in living Mealworm Larvae (Tenebrio molitor L.) Using near-infrared reflectance spectroscopy (NIRS). Insects. 13 (6): 560. doi: 10.3390/insects13060560
Lecrenier MC, Marbaix H, Dieu M, Veys P, Saegerman C, Raes M, Baeten V. 2016. Identification of specific bovine blood biomarkers with a non-targeted approach using HPLC ESI tandem mass spectrometry. Food Chem. 213: 417–424. doi: 10.1016/j.foodchem.2016.06.113
Lecrenier M-C, Marien A, Veys P, Belghit I, Dieu M, Gillard N, Henrottin J, Herfurth UM, Marchis D, Morello S, et al. 2021. Inter-laboratory study on the detection of bovine processed animal protein in feed by LC-MS/MS-based proteomics. Food Control. 125: 107944. doi: 10.1016/j.foodcont.2021.107944
Lecrenier MC, Planque M, Dieu M, Veys P, Saegerman C, Gillard N, Baeten V. 2018. A mass spectrometry method for sensitive, specific and simultaneous detection of bovine blood meal, blood products and milk products in compound feed. Food Chem. 245: 981–988. doi: 10.1016/j.foodchem.2017.11.074
Lecrenier MC, Veys P, Fumière O, Berben G, Saegerman C, Baeten V. 2020. Official feed control linked to the detection of animal by-products: past, present, and future. J Agric Food Chem. 68 (31): 8093–8103. doi: 10.1021/acs.jafc.0c02718
Mandrile L, Amato G, Marchis D, Martra G, Rossi AM. 2017. Species-specific detection of processed animal proteins in feed by Raman spectroscopy. Food Chem. 229: 268–275. doi: 10.1016/j.foodchem.2017.02.089
Mandrile L, Fusaro I, Amato G, Marchis D, Martra G, Rossi AM. 2018. Detection of insect’s meal in compound feed by near infrared spectral imaging. Food Chem. 267: 240–245. doi: 10.1016/j.foodchem.2018.01.127
Marbaix H, Budinger D, Dieu M, Fumière O, Gillard N, Delahaut P, Mauro S, Raes M. 2016. Identification of proteins and peptide biomarkers for detecting banned processed animal proteins (PAPs) in meat and bone meal by mass spectrometry. J Agric Food Chem. 64 (11): 2405–2414. doi: 10.1021/acs.jafc.6b00064
Marien A, Debode F, Aerts C, Ancion C, Francis F, Berben G. 2018. Detection of Hermetia illucens by real-time PCR. J Insects Food Feed. 4 (2): 115–122. 8). doi: 10.3920/JIFF2017.0069
Murray I, Aucott LS, Pike IH. 2001. Use of discriminant analysis on visible and near infrared reflectance spectra to detect adulteration of fishmeal with meat and bone meal. J Near Infrared Spectrosc. 9 (4): 297–311. doi: 10.1255/jnirs.315
Ocaña MF, Neubert H, Przyborowska A, Parker R, Bramley P, Halket J, Patel R. 2004. BSE Control: detection of gelatine-derived peptides in animal feed by mass spectrometry. Analyst. 129 (2): 111–115. doi: 10.1039/B312593A
Piraux F, Dardenne P. 2000. Feed authentication by near infrared microscopy. Near infrared spectroscopy: Proceedings of the 9th International Conference.:535.
Sánchez-Muros M-J, Barroso F, Manzano-Agugliaro F. 2014. Insect meal as renewable source of food for animal feeding: a review. J Cleaner Prod. 65: 16–27. doi: 10.1016/j.jclepro.2013.11.068
Sousa AH, Faroni LRD, Guedes RNC, Tótola MR, Urruchi WI. 2008. Ozone as a management alternative against phosphine-resistant insect pests of stored products. J Stored Prod Res. 44 (4): 379–385. doi: 10.1016/j.jspr.2008.06.003
Steinhilber A, Schmidt F, Naboulsi W, Planatscher H, Niedzwiecka A, Zagon J, Braeuning A, Lampen A, Joos T, Poetz O. 2018. Species differentiation and quantification of processed animal proteins and blood products in fish feed using an 8-plex mass spectrometry-based immunoassay. J Agric Food Chem. 66 (39): 10327–10335. doi: 10.1021/acs.jafc.8b03934
Steinhilber A, Schmidt F, Naboulsi W, Planatscher H, Niedzwiecka A, Zagon J, Braeuning A, Lampen A, Joos T, Poetz O. 2019. Application of mass spectrometry-based immunoassays for the species- and tissue-specific quantification of banned processed animal proteins in feeds. Anal Chem. 91 (6): 3902–3911. doi: 10.1021/acs.analchem.8b04652
Tena N, Fernández Pierna JA, Boix A, Baeten V, von Holst C. 2014. Differentiation of meat and bone meal from fishmeal by near-infrared spectroscopy: extension of scope to defatted samples. Food Control. 43: 155–162. doi: 10.1016/j.foodcont.2014.03.001
van Raamsdonk L, Zegers J, van Cutsem J, Bosch J, Pinckaers V, Jorgenson JS, Frick G, Paradies-Severin I. 2005. Microscopic detection of animal by-products in feed (WP3). In: Dardenne P, editor. Strategies and methods to detect and quantify mammalian tissues in feedingstuffs. Bruxelles (BE): European Commission. p. 16.
Veldkamp T, Bosch G. 2015. Insects: a protein-rich feed ingredient in pig and poultry diets. Anim Front. 5 (2): 6. doi: 10.2527/af.2015-0019
Veys P, Baeten V. 2018. Protocol for the isolation of processed animal proteins from insects in feed and their identification by microscopy. Food Control. 92: 496–504. doi: 10.1016/j.foodcont.2018.05.028
Veys P, Baeten V, Berben G. 2019. Validation study on the isolation of insect PAP in feed by double sedimentation method PE/TCE and subsequent detection by light microscopy. [accessed 2019 Oct 23]. https://www.eurl.craw.eu/wp-content/uploads/2019/10/report_insect_study_final.pdf.
Veys P, Berben G, Baeten V. 2009. CRL-AP Proficiency Test 2009. [accessed 2012 May 1]. https://www.eurl.craw.eu/wp-content/uploads/2012/05/20120524997bfeb6.pdf.
Veys P, Berben G, Dardenne P, Baeten V. 2012. Detection and identification of animal by-products in animal feed for the control of transmissible spongiform encephalopathies. In: Fink-Gremmels J, editor. Woodhead Publishing Series in Food Science, Technology and Nutrition, Animal Feed Contamination - Effects on Livestock and Food Safety. Woodhead Publishing; p. 94–113. doi: 10.1533/9780857093615.1.94
von Holst C, Boix A, Baeten V, Vancutsem J, Berben G. 2006. Determination of processed animal proteins in feed: the performance characteristics of classical microscopy and immunoassay methods. Food Addit Contam. 23 (3): 252–264. doi: 10.1080/02652030500471804
Wardhana AH. 2017. Black Soldier Fly (Hermetia illucens) as an Alternative Protein Source for Animal Feed. WARTAZOA. 26 (2): 069. doi: 10.14334/wartazoa.v26i2.1327
Zagon J, di Rienzo V, Potkura J, Lampen A, Braeuning A. 2018. A real-time PCR method for the detection of black soldier fly (Hermetia illucens) in feedstuff. Food Control. 91: 440–448. doi: 10.1016/j.foodcont.2018.04.032