Chenopodiaceae; deep eutectic solvent-storage stabilization; flavonoids green extraction; natural radical quenching; Deep eutectic solvent-storage stabilization; Deep eutectic solvents; Flavonoid; Flavonoid green extraction; Green extractions; Natural radical quenching; Radical scavengers; Radical-scavenging activities; Analytical Chemistry; Filtration and Separation
Abstract :
[en] Flavonoids are probable major contributors to the radical scavenging activity in Ecuadorian quinoa leaves, both from the bitter genotype (Chimborazo) and sweet varieties. In this study, we extracted these compounds using a simple, rapid (10 min), and environmentally friendly method based on deep eutectic solvents (DES). Extractions were performed in a ball mixer mill at room temperature with a eutectic mixture of choline chloride—glycerol—water at a molar ratio, of 1:2:1 and compared with classical methanol extraction. Both extracts were characterized using high-performance thin-layer chromatography and liquid chromatography-tandem mass spectrometry-based methods. Regardless of the type of solvent used (conventional or green solvent), quercetin and kaempferol glycosides were found as the major flavonols in sweet and bitter quinoa leaves. DES extract contains a higher amount of quercetin glycosides than methanol and shows a higher capacity to stabilize the quinoa radical scavengers compared to conventional solvent (liquids extracts—conservation for up to 4 months at 5°C). The present research indicates that DES represent an efficient green media for the stabilization of phenolic compounds from quinoa leaves and has the potential as a possible alternative to organic solvents. Our work opens new perspectives for the development of high-added value products based on quinoa leaves for pharmaceutical, nutraceutical and agro-food applications.
Taco, Verónica; Faculty of Chemical Sciences, Central University of Ecuador, Quito, Ecuador ; Unit of Therapeutic Chemistry and Pharmacognosy, Faculty of Medicine and Pharmacy, University of Mons, Mons, Belgium
Semay, Irène ; Université de Mons - UMONS > Faculté des Sciences > Service de Synthèse et spectrométrie de masse organiques
Villacrés, Elena; Departamento de Nutrición y Calidad, Instituto Nacional de Investigaciones Agropecuarias (INIAP), Mejia, Ecuador
Santamaría, Javier; Faculty of Chemical Sciences, Central University of Ecuador, Quito, Ecuador
Flores, Ronny; Faculty of Chemical Sciences, Central University of Ecuador, Quito, Ecuador
Gerbaux, Pascal ; Université de Mons - UMONS > Faculté des Sciences > Service de Synthèse et spectrométrie de masse organiques
Duez, Pierre ; Université de Mons - UMONS > Faculté de Médecine, Pharmacie et Sciences Biomédicales > Service de Chimie thérapeutique et Pharmacognosie
Nachtergael, Amandine ; Université de Mons - UMONS > Faculté de Médecine et de Pharmacie > Service de Chimie thérapeutique et Pharmacognosie
Language :
English
Title :
Deep Eutectic Solvents for the Extraction and Stabilization of Radical Scavengers From Ecuadorian Quinoa (Chenopodium quinoa Willd.) Leaves
Publication date :
14 April 2025
Journal title :
Separation Science Plus
eISSN :
2573-1815
Publisher :
John Wiley and Sons Inc
Volume :
8
Issue :
4
Peer reviewed :
Peer Reviewed verified by ORBi
Research unit :
S836 - Synthèse et spectrométrie de masse organiques M136 - Chimie thérapeutique et Pharmacognosie
Research institute :
R100 - Institut des Biosciences R550 - Institut des Sciences et Technologies de la Santé
Funding text :
The project Assessment of the bioactive properties of the hydrolyzed peptides and phenolic compounds of the leaves of quinoa (Chenopodium quinoa Willd.), INIAP Tunkahuan variety with potential use in the food and pharmaceutical industries was funded by the Académie de recherche et d'enseignement supérieur de la Fédération Wallonie-Bruxelles (ARES CCD) through the ARES AI Programme at the Central University of Ecuador. V. Taco gratefully acknowledges the fellowship granted to her by ARES. The S2MOs laboratory is grateful to the Fonds de la Recherche Scientifique (FNRS) for financial support for the acquisition of the Waters Synapt G2-Si mass spectrometer. The PhD grant of I. Semay is supported by the \u00AB Action de Recherche Concert\u00E9e \u00BB (ARC) project METAFLORE.The project \u201CAssessment of the bioactive properties of the hydrolyzed peptides and phenolic compounds of the leaves of quinoa (Chenopodium quinoa Willd), INIAP Tunkahuan variety with potential use in the food and pharmaceutical industries\u201D was funded by the Acad\u00E9mie de recherche et d'enseignement sup\u00E9rieur\u00A0de la F\u00E9d\u00E9ration Wallonie-Bruxelles (ARES CCD) through the ARES AI Programme at the Central University of Ecuador. V. Taco gratefully acknowledges the fellowship granted to her by ARES. The S2MOs laboratory is grateful to the Fonds de la Recherche Scientifique (FNRS) for financial support for the acquisition of the Waters Synapt G2-Si mass spectrometer. The PhD grant of I. Semay is supported by the \u00AB Action de Recherche Concert\u00E9e \u00BB (ARC) project METAFLORE. We would like to thank the Maquita Cushunchic (MCCH) company in Ecuador for providing the quinoa leaf samples for this study. We thank Ra\u00FAl L\u00F3pez, Claudio Palmieri, Ixchel Gij\u00F3n-Arreort\u00FAa, and Benjam\u00EDn Mart\u00EDnez for their help and support.The project \u201C Willd.\u201D was funded by the Acad\u00E9mie de recherche et d'enseignement sup\u00E9rieur de la F\u00E9d\u00E9ration Wallonie\u2010Bruxelles (ARES CCD) through the ARES AI Programme at the Central University of Ecuador. V. Taco gratefully acknowledges the fellowship granted to her by ARES. The SMOs laboratory is grateful to the Fonds de la Recherche Scientifique (FNRS) for financial support for the acquisition of the Waters Synapt G2-Si mass spectrometer. The PhD grant of I. Semay is supported by the "Action de Recherche Concertée" (ARC) project METAFLORE. Funding: Assessment of the bioactive properties of the hydrolyzed peptides and phenolic compounds of the leaves of quinoa (Chenopodium quinoa ), INIAP Tunkahuan variety with potential use in the food and pharmaceutical industries
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